Wild-type (C57BL/6), age day 6 (P6) genotype: WT; Tissue: cerebellum; Age: P6
Growth protocol
Standard mouse facility
Extracted molecule
total RNA
Extraction protocol
Total RNA extraction by Trizol
Library strategy
RNA-Seq
Library source
transcriptomic
Library selection
size fractionation
Instrument model
Illumina Genome Analyzer
Description
Barcode used in pool: 5MLD Raw data file: CSHL_FC9506_4_Medullo_5.fastq
Data processing
The reads were deconvoluted by barcode, collapsed to unique sequences, aligned to the genome and annotated. Read-counts for miRNA sequences were cubic-spline normalized then log-transformed. The R-project software (http://www.R-project.org), including Bioconductor were used for data analysis.