Barcode used in pool: 5MLC Raw data file: CSHL_FC12772_5_Medullo_4.fastq
Data processing
The reads were deconvoluted by barcode, collapsed to unique sequences, aligned to the genome and annotated. Read-counts for miRNA sequences were cubic-spline normalized then log-transformed. The R-project software (http://www.R-project.org), including Bioconductor were used for data analysis.