|
| Status |
Public on Feb 22, 2019 |
| Title |
hBMMSCs printed with CdECM bioink 2 |
| Sample type |
RNA |
| |
|
| Source name |
3D cell-printed construct (pre-tissue)
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: human bone marrow derived mesenchymal stem cells printed with Cornea dECM bioink 2, 3D cell-printed construct, 7 days culture
|
| Treatment protocol |
After 80-90% confluence, hBMMSCs were detached using 0.05% trypsin-EDTA (Sigma-Aldrich, USA), and encapsulated in 1.5% (w/v) of liver, heart, skin and cornea dECM bioink (5×10^6 cells/ml). Then, the cell-laden constructs were printed using an in-house-developed 3D cell printing system. Cell-printed constructs were incubated at 37°C for 30 minutes to induce thermal crosslinking. The cell-printed constructs were cultured for seven days in the same culture media condition.
|
| Growth protocol |
Human bone marrow derived mesenchymal stem cells (hBMMSCs) (Cefobio, Korea) were cultured using DMEM/F12 (1:1) from Gibco with 10% fetal bovine serum (FBS) (Gibco, USA) and 1% penicillin/streptomycin (Gibco, USA)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
The sense cDNA was then fragmented and biotin-labeled with TdT (terminal deoxynucleotidyl transferase) using the GeneChip WT Terminal labeling kit
|
| |
|
| Hybridization protocol |
Approximately 5.5 μg of labeled DNA target was hybridized to the Affymetrix GeneChip Array at 45°C for 16hour
|
| Scan protocol |
Hybridized arrays were washed and stained on a GeneChip Fluidics Station 450 and scanned on a GCS3000 Scanner (Affymetrix)
|
| Description |
Gene expression data from hBMMSCs printed with CdECM bioink 2
|
| Data processing |
Array data export processing and analysis was performed using Affymetrix® GeneChip Command Console® Software (AGCC). Data processed with RMA.
|
| |
|
| Submission date |
Feb 21, 2019 |
| Last update date |
Feb 22, 2019 |
| Contact name |
Dong-woo Cho |
| E-mail(s) |
dwcho@postech.ac.kr
|
| Organization name |
POSTECH
|
| Department |
Department of Mechanical Engineering
|
| Street address |
77 Cheongam-ro, Nam-gu
|
| City |
Pohang |
| State/province |
Gyeongbuk |
| ZIP/Postal code |
37673 |
| Country |
South Korea |
| |
|
| Platform ID |
GPL23159 |
| Series (1) |
| GSE126877 |
Expression data from 3D cell-printed human bone marrow derived mesenchymal stem cells (hBMMSCs) with liver, heart, skin and cornea decellularized extracellular matrix (dECM) bioinks |
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