Country of origin: India, Internal identifier number: 248-97, SIV status: negative, Tissue: peripheral blood.
Treatment protocol
normal animal
Extracted molecule
total RNA
Extraction protocol
FACS-isolated monocytes were spun down and lysed in Trizol reagents (Invitrogen), and total RNA was prepared using PureLink Micro-to-Midi Total RNA Purification system (Invitrogen). Quality of RNA was determined by 2100 Bioanalyzer RNA LabChip (Agilent Technologies).
Label
biotin
Label protocol
Standard Affymetrix protocol, processed at Pathologica, LLC
Hybridization protocol
Standard Affymetrix protocol, processed at Pathologica, LLC
Scan protocol
Standard Affymetrix protocol, processed at Pathologica, LLC
Description
The transcriptional profile in the CD14+ monocyte subset of a rhesus monkey was examined using the Affymetrix GeneChip Rhesus Macaque Genome Array containing a total of 47,000 Macaca mulatta transcripts
Data processing
Expression data was obtained using an Affymetrix GSC3000 scanner and processed by GCOS software (Affymetrix). Partek Genomic Suite System was used for downstream analysis of GCOS processed data.
