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Sample GSM361843 Query DataSets for GSM361843
Status Public on Jan 31, 2009
Title Monocytes_CD14_CD16_uninfected1
Sample type RNA
 
Source name FACS sorted CD14+CD16+ monocytes
Organism Macaca mulatta
Characteristics Country of origin: India,
Internal identifier number: 248-97,
SIV status: negative,
Tissue: peripheral blood.
Treatment protocol normal animal
Extracted molecule total RNA
Extraction protocol FACS-isolated monocytes were spun down and lysed in Trizol reagents (Invitrogen), and total RNA was prepared using PureLink Micro-to-Midi Total RNA Purification system (Invitrogen). Quality of RNA was determined by 2100 Bioanalyzer RNA LabChip (Agilent Technologies).
Label biotin
Label protocol Standard Affymetrix protocol, processed at Pathologica, LLC
 
Hybridization protocol Standard Affymetrix protocol, processed at Pathologica, LLC
Scan protocol Standard Affymetrix protocol, processed at Pathologica, LLC
Description The transcriptional profile in the CD14+CD16+ monocyte subset of a rhesus monkey was examined using the Affymetrix GeneChip Rhesus Macaque Genome Array containing a total of 47,000 Macaca mulatta transcripts
Data processing Expression data was obtained using an Affymetrix GSC3000 scanner and processed by GCOS software (Affymetrix). Partek Genomic Suite System was used for downstream analysis of GCOS processed data.
 
Submission date Jan 20, 2009
Last update date Jan 22, 2009
Contact name K C Williams
E-mail(s) williauy@bc.edu
Phone 617-552-1186
Organization name Boston College
Department Biology
Lab Williams
Street address 140 Commonwealth Avenue
City Chestnut Hill
State/province MA
ZIP/Postal code 02467
Country USA
 
Platform ID GPL3535
Series (1)
GSE14482 Differentially expressed genes in subpopulations of monocytes from non infected animals.

Data table header descriptions
ID_REF
VALUE signal count data normalized using Rhesus Array Normalization Controls
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or reverse present (RP)
DETECTION P-VALUE p-value that indicates the significance level of the detection call

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-BioB-5_at 105.098 P 0.000753643
AFFX-BioB-M_at 164.57 P 4.42873e-05
AFFX-BioB-3_at 86.077 P 0.000169227
AFFX-BioC-5_at 292.964 P 5.16732e-05
AFFX-BioC-3_at 317.395 P 5.16732e-05
AFFX-BioDn-5_at 802.352 P 4.42873e-05
AFFX-BioDn-3_at 1370.9 P 4.42873e-05
AFFX-CreX-5_at 4001.54 P 4.42873e-05
AFFX-CreX-3_at 4902.08 P 4.42873e-05
AFFX-DapX-5_at 55.8732 P 4.42873e-05
AFFX-DapX-M_at 641.882 P 6.02111e-05
AFFX-DapX-3_at 1544.24 P 5.16732e-05
AFFX-LysX-5_at 18.0967 A 0.083592
AFFX-LysX-M_at 103.271 P 0.000258358
AFFX-LysX-3_at 292.853 P 4.42873e-05
AFFX-PheX-5_at 16.1672 P 0.0200219
AFFX-PheX-M_at 61.6645 P 0.00179591
AFFX-PheX-3_at 131.274 P 5.16732e-05
AFFX-ThrX-5_at 8.95504 A 0.313723
AFFX-ThrX-M_at 141.305 P 0.000169227

Total number of rows: 52865

Table truncated, full table size 2125 Kbytes.




Supplementary file Size Download File type/resource
GSM361843.CEL.gz 4.8 Mb (ftp)(http) CEL
GSM361843.CHP.gz 301.4 Kb (ftp)(http) CHP
Processed data included within Sample table
Processed data provided as supplementary file

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