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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Jan 21, 2009 |
| Title |
iTreg-H3K27me3 |
| Sample type |
SRA |
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| Source name |
iTreg cells
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| Organism |
Mus musculus |
| Characteristics |
iTreg-H3K27me3
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| Treatment protocol |
Treatment protocol as described in Wei et al., 2009 (PMID:19144320)
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| Growth protocol |
Growth protocol as described in Wei et al., 2009 (PMID:19144320)
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| Extracted molecule |
genomic DNA |
| Extraction protocol |
Extract protocol as described in Wei et al., 2009 (PMID:19144320)
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| Library strategy |
ChIP-Seq |
| Library source |
genomic |
| Library selection |
ChIP |
| Instrument model |
Illumina Genome Analyzer |
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| Description |
Chromatin IP
T cells were treated with MNase to generate approximately 80% mononucleosomes and 20% dinucleosomes. Chromatin from 2 × 107 cells was used for each ChIP experiment, which yielded approximately 100 ng of DNA. Antibodies against histone H3K4me3 (ab8580, Abcam) and H3K27me3 (07-449, Upstate) were used. The ChIP DNA fragments were blunt-ended, ligated to the Solexa adaptors, and sequenced with the Illumina 1G Genome Analyzer.
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| Data processing |
Sequence reads were obtained and mapped to the mouse (February, 2006) genomes using the Illumina Genome Analyzer Pipeline. More details as described in Wei et al., 2009 (PMID:19144320)
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| Submission date |
Jan 21, 2009 |
| Last update date |
May 15, 2019 |
| Contact name |
Weiqun Peng |
| E-mail(s) |
wpeng@gwu.edu
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| Organization name |
The Georege Washington University
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| Street address |
725 21st street, NW
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| City |
DC |
| ZIP/Postal code |
20052 |
| Country |
USA |
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| Platform ID |
GPL9185 |
| Series (1) |
| GSE14254 |
Global Mapping of Histone H3 K4 and K27 Trimethylation: Lineage Fate Determination of Differentiating CD4+ T Cells |
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| Relations |
| SRA |
SRX003805 |
| BioSample |
SAMN02195442 |
| Named Annotation |
GSM362006.bed.gz |
| Supplementary file |
Size |
Download |
File type/resource |
| GSM362006.bed.gz |
95.3 Mb |
(ftp)(http) |
BED |
SRA Run Selector |
| Processed data provided as supplementary file |
| Raw data are available in SRA |
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