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Status |
Public on Feb 04, 2009 |
Title |
Müller glia at 16 hpl, biological rep1 |
Sample type |
RNA |
|
|
Source name |
zebrafish Müller glia isolated from 16 hour post-lesion retinas
|
Organism |
Danio rerio |
Characteristics |
zebrafish line: Tg(gfap:GFP)mi2002 age: 5- to 6-month old
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was extracted using the RNAqueous-4PCR kit (Ambion).
|
Label |
biotin
|
Label protocol |
20 ng of total RNA was used for linear amplification and biotin-labeling with Ovation Biotin Labeling System (NuGEN).
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|
|
Hybridization protocol |
2.75 µg of biotin-labeled, fragmented cDNA was hybridized to a GeneChip Zebrafish Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
|
Description |
Gene expression data of zebrafish Müller glia isolated from 16 hour post-lesion retinas
|
Data processing |
The Robust Multichip Average (RMA) method was used for background correction, quantile normalization, and summarization of expression scores.
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|
|
Submission date |
Jan 21, 2009 |
Last update date |
Feb 03, 2009 |
Contact name |
Zhao Qin |
E-mail(s) |
qinzhao@umich.edu
|
Phone |
734-764-7476
|
Organization name |
University of Michigan
|
Department |
Molecular, Cellular and Developmental Biology
|
Street address |
830 North University
|
City |
Ann Arbor |
State/province |
MI |
ZIP/Postal code |
48109 |
Country |
USA |
|
|
Platform ID |
GPL1319 |
Series (1) |
GSE14495 |
Gene profiling of Müller glia during early stages of zebrafish photoreceptor regeneration |
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