|
| Status |
Public on Feb 04, 2009 |
| Title |
Müller glia at 24 hpl, biological rep2 |
| Sample type |
RNA |
| |
|
| Source name |
zebrafish Müller glia isolated from 24 hour post-lesion retinas
|
| Organism |
Danio rerio |
| Characteristics |
zebrafish line: Tg(gfap:GFP)mi2002
age: 5- to 6-month old
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using the RNAqueous-4PCR kit (Ambion).
|
| Label |
biotin
|
| Label protocol |
20 ng of total RNA was used for linear amplification and biotin-labeling with Ovation Biotin Labeling System (NuGEN).
|
| |
|
| Hybridization protocol |
2.75 µg of biotin-labeled, fragmented cDNA was hybridized to a GeneChip Zebrafish Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
|
| Description |
Gene expression data of zebrafish Müller glia isolated from 24 hour post-lesion retinas
|
| Data processing |
The Robust Multichip Average (RMA) method was used for background correction, quantile normalization, and summarization of expression scores.
|
| |
|
| Submission date |
Jan 21, 2009 |
| Last update date |
Feb 03, 2009 |
| Contact name |
Zhao Qin |
| E-mail(s) |
qinzhao@umich.edu
|
| Phone |
734-764-7476
|
| Organization name |
University of Michigan
|
| Department |
Molecular, Cellular and Developmental Biology
|
| Street address |
830 North University
|
| City |
Ann Arbor |
| State/province |
MI |
| ZIP/Postal code |
48109 |
| Country |
USA |
| |
|
| Platform ID |
GPL1319 |
| Series (1) |
| GSE14495 |
Gene profiling of Müller glia during early stages of zebrafish photoreceptor regeneration |
|
