|
| Status |
Public on Sep 08, 2009 |
| Title |
liver Wrn mutant #2 / liver WT #2 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
liver from wild type animal
|
| Organism |
Mus musculus |
| Characteristics |
Strain: C57Bl/6
|
| Biomaterial provider |
Laboratory of Michel Lebel, Centre de recherche en cancérologie, Hôpital Hôtel-Dieu de Québec, Québec City, Canada
|
| Growth protocol |
Mice were maintained on a reversed 12:12-h light-dark cycle with controlled temperature (21 ± 1°C) and humidity. Food and water were provided ad libitum.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Sambrook et al., 1989. Molecular cloning: A Laboratory Manual, Cold Spring Harbor Laboratory Press.
|
| Label |
Cy3
|
| Label protocol |
Protocol from Agilent
|
| |
|
| Channel 2 |
| Source name |
liver from Wrn mutant
|
| Organism |
Mus musculus |
| Characteristics |
Strain: C57Bl/6
|
| Biomaterial provider |
Laboratory of Michel Lebel, Centre de recherche en cancérologie, Hôpital Hôtel-Dieu de Québec, Québec City, Canada
|
| Growth protocol |
Mice were maintained on a reversed 12:12-h light-dark cycle with controlled temperature (21 ± 1°C) and humidity. Food and water were provided ad libitum.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Sambrook et al., 1989. Molecular cloning: A Laboratory Manual, Cold Spring Harbor Laboratory Press.
|
| Label |
Cy5
|
| Label protocol |
Protocol from Agilent
|
| |
|
| |
| Hybridization protocol |
Hybridization and washing was performed using the in situ Hybridization Plus kit (Agilent) and following the manufacturer's instructions.
|
| Scan protocol |
Arrays were scanned on an Axon GenePix® 4000B. Data were extracted from images using GenePix® version 6.0 with local background estimation.
|
| Description |
WT #2 vs. Wrn mutant #2
|
| Data processing |
Data were processed using limma in BioConductor (www.bioconductor.org). The data were background subtracted using normexp and also normalized using the loess method.
|
| |
|
| Submission date |
Jan 23, 2009 |
| Last update date |
Sep 08, 2009 |
| Contact name |
Eric R Paquet |
| Organization name |
EPFL
|
| Department |
Life sciences
|
| Lab |
Naef lab
|
| Street address |
Station 15 CH - 1015
|
| City |
Lausanne |
| State/province |
Vaud |
| ZIP/Postal code |
1015 |
| Country |
Switzerland |
| |
|
| Platform ID |
GPL4134 |
| Series (1) |
| GSE14549 |
Gene expression profiling in liver of mice with a mutant Wrn protein treated with/without vitamin C compared to WT mice. |
|
