|
| Status |
Public on Dec 28, 2009 |
| Title |
Ovine GW9662 2 |
| Sample type |
RNA |
| |
|
| Source name |
PAEC
|
| Organism |
Ovis aries |
| Characteristics |
GW9662 treated
|
| Treatment protocol |
Ovine PAECs were treated with vehicle (DMSO) or 5μM GW9662 for 24 hours
|
| Growth protocol |
10% FBS-DMEM
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using the Qiagen Rneasy Mini kit.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Fragmented cRNA was hybridized for 16 h at 45°C to the array chips (Affymetrix). Genechips were washed ans stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
n/a
|
| Data processing |
Microarray data were processed and normalized using RMA.
|
| |
|
| Submission date |
Jan 23, 2009 |
| Last update date |
Jan 23, 2009 |
| Contact name |
Jing Tian |
| E-mail(s) |
jtian@mcg.edu
|
| Phone |
706-721-0074
|
| Organization name |
Medical College of Georgia
|
| Department |
Vascular Biology Center
|
| Lab |
Lab of Stephen M. Black
|
| Street address |
1459 Laney Walker BLVD
|
| City |
Augusta |
| State/province |
GA |
| ZIP/Postal code |
30912 |
| Country |
USA |
| |
|
| Platform ID |
GPL2112 |
| Series (1) |
| GSE14546 |
Effect of PPARγ inhibition on pulmonary endothelial cell gene expression: Gene profiling in pulmonary hypertension |
|
