|
Status |
Public on Dec 28, 2009 |
Title |
Ovine GW9662 4 |
Sample type |
RNA |
|
|
Source name |
PAEC
|
Organism |
Ovis aries |
Characteristics |
GW9662 treated
|
Treatment protocol |
Ovine PAECs were treated with vehicle (DMSO) or 5μM GW9662 for 24 hours
|
Growth protocol |
10% FBS-DMEM
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was extracted using the Qiagen Rneasy Mini kit.
|
Label |
biotin
|
Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
|
|
Hybridization protocol |
Fragmented cRNA was hybridized for 16 h at 45°C to the array chips (Affymetrix). Genechips were washed ans stained in the Affymetrix Fluidics Station 400.
|
Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
Description |
n/a
|
Data processing |
Microarray data were processed and normalized using RMA.
|
|
|
Submission date |
Jan 23, 2009 |
Last update date |
Jan 23, 2009 |
Contact name |
Jing Tian |
E-mail(s) |
jtian@mcg.edu
|
Phone |
706-721-0074
|
Organization name |
Medical College of Georgia
|
Department |
Vascular Biology Center
|
Lab |
Lab of Stephen M. Black
|
Street address |
1459 Laney Walker BLVD
|
City |
Augusta |
State/province |
GA |
ZIP/Postal code |
30912 |
Country |
USA |
|
|
Platform ID |
GPL2112 |
Series (1) |
GSE14546 |
Effect of PPARγ inhibition on pulmonary endothelial cell gene expression: Gene profiling in pulmonary hypertension |
|