|
| Status |
Public on Feb 26, 2009 |
| Title |
Tel_Fad_Control1 |
| Sample type |
RNA |
| |
|
| Source name |
Danio rerio - Telencephalon - control (slide 2509)
|
| Organism |
Danio rerio |
| Characteristics |
tissue: Telencephalon
developmental stage: Sexually mature males
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNeasy Mini (qiagen)
|
| Label |
Biotin
|
| Label protocol |
Ten micrograms of each RNA sample was labeled using the Super Script II (Invitrogen) and the Enzo BioArray HighYield RNA transcript labeling kit (Affymetrix).
|
| |
|
| Hybridization protocol |
Labeled complementary RNA was hybridized to the Affymetrix microarrays according to the specifications of the manufacturer (Affymetrix) at the Affymetrix gene expression facility at the Ottawa Hospital Research Institute (Ottawa).
|
| Scan protocol |
Scanning was done according to the specifications of the manufacturer (Affymetrix) at the Affymetrix gene expression facility at the Ottawa Hospital Research Institute (Ottawa).
|
| Description |
Control 1
|
| Data processing |
Analysis was done with Genespring GX (Agilent). Data was normalized by: (1) setting expression measurements < 0.01 to 0.01, (2) per chip normalization to the 50th percentile, and (3) per gene normalization to specific samples.
|
| |
|
| Submission date |
Jan 26, 2009 |
| Last update date |
Feb 26, 2009 |
| Contact name |
Martin Paquette |
| Organization name |
University of Ottawa
|
| Department |
Department of Biology
|
| Lab |
Center for Advanced Research in Environmental Genomics
|
| Street address |
30 Marie Curie
|
| City |
Ottawa |
| State/province |
Ontario |
| ZIP/Postal code |
K1N 6N5 |
| Country |
Canada |
| |
|
| Platform ID |
GPL1319 |
| Series (1) |
| GSE14718 |
Effects of the Aromatase Inhibitor Fadrozole on Gene Expression in the Zebrafish Telencephalon |
|
