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Sample GSM3664833 Query DataSets for GSM3664833
Status Public on Apr 03, 2019
Title Case 1
Sample type SRA
 
Source name rat knee joints treated with BNTA (1.5 mg kg-1) twice a week for 4 w
Organism Rattus norvegicus
Characteristics tissue: Cartilage tissue of rat knee joints
Treatment protocol Cartilage tissues were collected from medial and lateral femoral condyles of anterior cruciate ligament transection-operated rat knee joints treated with BNTA (1.5 mg kg-1) twice a week for 4 w.
Extracted molecule polyA RNA
Extraction protocol RNA was harvested using Trizol reagent.
RNA libraries were prepared for sequencing using standard Illumina protocols
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model HiSeq X Ten
 
Data processing Illumina Casava1.7 software used for basecalling.
Reads filtering under criteria removing reads with 20% of the base quality lower than 13
HISAT2.1.0 mapping to reference genome
count counting
Genome_build: Rnor_6.0
Supplementary_files_format_and_content: tab-delimited text files include FPKM values for each Sample
 
Submission date Mar 11, 2019
Last update date Apr 03, 2019
Contact name shi yuanyuan
E-mail(s) shiyuanyuan815@163.com
Organization name Peking University
Street address No.5 Yiheyuan Road Haidian District, Beijing, P.R.China
City Beijing
State/province Bejing
ZIP/Postal code 100871
Country China
 
Platform ID GPL24688
Series (1)
GSE128093 A small molecule promotes cartilage extracellular matrix generation and inhibits osteoarthritis development
Relations
BioSample SAMN11098935
SRA SRX5502819

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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