|
Status |
Public on Apr 03, 2019 |
Title |
Case 1 |
Sample type |
SRA |
|
|
Source name |
rat knee joints treated with BNTA (1.5 mg kg-1) twice a week for 4 w
|
Organism |
Rattus norvegicus |
Characteristics |
tissue: Cartilage tissue of rat knee joints
|
Treatment protocol |
Cartilage tissues were collected from medial and lateral femoral condyles of anterior cruciate ligament transection-operated rat knee joints treated with BNTA (1.5 mg kg-1) twice a week for 4 w.
|
Extracted molecule |
polyA RNA |
Extraction protocol |
RNA was harvested using Trizol reagent. RNA libraries were prepared for sequencing using standard Illumina protocols
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
HiSeq X Ten |
|
|
Data processing |
Illumina Casava1.7 software used for basecalling. Reads filtering under criteria removing reads with 20% of the base quality lower than 13 HISAT2.1.0 mapping to reference genome count counting Genome_build: Rnor_6.0 Supplementary_files_format_and_content: tab-delimited text files include FPKM values for each Sample
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|
|
Submission date |
Mar 11, 2019 |
Last update date |
Apr 03, 2019 |
Contact name |
shi yuanyuan |
E-mail(s) |
shiyuanyuan815@163.com
|
Organization name |
Peking University
|
Street address |
No.5 Yiheyuan Road Haidian District, Beijing, P.R.China
|
City |
Beijing |
State/province |
Bejing |
ZIP/Postal code |
100871 |
Country |
China |
|
|
Platform ID |
GPL24688 |
Series (1) |
GSE128093 |
A small molecule promotes cartilage extracellular matrix generation and inhibits osteoarthritis development |
|
Relations |
BioSample |
SAMN11098935 |
SRA |
SRX5502819 |