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Sample GSM368071 Query DataSets for GSM368071
Status Public on Feb 07, 2009
Title retina_time 0_CD133+_donor1
Sample type RNA
 
Source name CD133+
Organism Homo sapiens
Characteristics tissue: retina
Treatment protocol Retina was dissociated to form a single cell population and CD133+ & CD133- cells were isolated using magnetic beads
Growth protocol human retina was disected from post mortem eyes (with research consent)
Extracted molecule total RNA
Extraction protocol 300ng of RNA were isolated from magnetically sorted cells using RNeasy mini® kit according to the manufacturers instructions
Label biotin
Label protocol GeneChip Whole Transcript (WT) sense target labeling assay manual, version 4 - starting with 300ng RNA and following the Total RNA labeling protocol to prepare targets for GeneChip gene 1.0 ST Arrays(omitting RiboMinus procedure).
 
Hybridization protocol using the genechip hybridisation, wash and stain kit
Scan protocol Array washing and staining as described in chp 6 of above manual using the GeneChip Fluidics station 450 (fluidics script FS450-007). Arrays were scanned on a GeneChip scanner 3000 7G using default scanner settings. Affymetrix GeneChip® Command Console® (AGCC) was used to process all Affymetrix arrays through fluidics and scanning.
Description DC_1-time0_(HuGene-1_0-st-v1).CEL
Data processing Gene level probe set summarisation (signal value), calculation of QC metrics and CHP file generation were performed using GeneChip® Expression Console software using the Robust Multichip Analysis (RMA) algorithm
 
Submission date Feb 06, 2009
Last update date Feb 06, 2009
Contact name DEBRA ANN CARTER
E-mail(s) debbie.carter@bristol.ac.uk
Organization name BRISTOL UNIVERSITY
Department CLINICAL SCIENCE @ SOUTH BRISTOL
Lab OPHTHALMOLOGY
Street address BRISTOL EYE HOSPITAL, LOWER MAUDLIN STREET
City BRISTOL
ZIP/Postal code BS1 2LX
Country United Kingdom
 
Platform ID GPL6244
Series (1)
GSE14733 Understanding adult human progenitor cells

Data table header descriptions
ID_REF
VALUE RMA

Data table
ID_REF VALUE
7896736 7.039258
7896738 4.607359
7896740 5.004553
7896742 8.451535
7896744 6.467049
7896746 9.54212
7896748 9.787083
7896750 4.782738
7896752 8.978415
7896754 8.548723
7896756 6.817118
7896759 7.930921
7896761 9.167155
7896779 7.688635
7896798 7.990453
7896817 7.437591
7896822 8.097876
7896859 7.068675
7896861 5.6063
7896863 6.664132

Total number of rows: 33297

Table truncated, full table size 549 Kbytes.




Supplementary file Size Download File type/resource
GSM368071.CEL.gz 4.3 Mb (ftp)(http) CEL
GSM368071.chp.gz 256.0 Kb (ftp)(http) CHP
Processed data included within Sample table
Processed data provided as supplementary file

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