|
| Status |
Public on Feb 09, 2010 |
| Title |
IM9 cells - ch128.1Av - 3h - rep1 |
| Sample type |
RNA |
| |
|
| Source name |
IM9 cells (sensitive) - ch128.1Av - 3h - rep1
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: IM9 (sensitive)
|
| Treatment protocol |
Treatment with ch128.1Av
2x10e5 IM-9 and cell line: U266 (tolerant) were incubated in 24-well plates under tissue culture conditions (37ºC and 5%CO2) with 10 nM ch128.1Av or buffer for 1h, 3h, 9h, and 24h
|
| Growth protocol |
Cells were grown in 24-well plates under tissue culture conditions (37ºC and 5%CO2)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA isolation as previously described (Pattenden et al., EMBO (2002) 21: 1978). Targets were prepared from 200 ng total RNA using Illumina. TotalPrep RNA Amplification Kit (Ambion, catalog #AMIL1791).
|
| Label |
Biotin
|
| Label protocol |
Illumina protocol
|
| |
|
| Hybridization protocol |
Illumina HumanRef-8 V2 BeadChips were hybridized, washed, stained and scanned according to Illumina’s protocol using the updated wash procedure
|
| Scan protocol |
Processed arrays were read using Illumina BeadReader according to the manufacturer's instructions.
|
| Description |
Time course experiment
Replicate 1/3
IM9AT3R1
|
| Data processing |
Data was extracted with Illumina BeadStudio software.
|
| |
|
| Submission date |
Feb 09, 2009 |
| Last update date |
Feb 09, 2009 |
| Contact name |
Manuel Penichet |
| E-mail(s) |
penichet@mednet.ucla.edu
|
| Phone |
+1-310-825-1304
|
| Fax |
+1-310-825-7575
|
| Organization name |
University of California, Los Angeles
|
| Department |
Department of Surgery
|
| Lab |
Division of Surgical Oncology
|
| Street address |
Box 951782
|
| City |
Los Angeles |
| State/province |
CA |
| ZIP/Postal code |
90095-1782 |
| Country |
USA |
| |
|
| Platform ID |
GPL6255 |
| Series (1) |
| GSE14754 |
Mechanism of sensitivity and resistance to treatment with an TfR1 antibody fusion protein in B-cell malignancies |
|
