NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM368563 Query DataSets for GSM368563
Status Public on Feb 09, 2010
Title cell line: U266 - ch128.1Av - 24h - rep1
Sample type RNA
 
Source name cell line: U266 (tolerant) - ch128.1Av - 24h - rep1
Organism Homo sapiens
Characteristics cell line: U266 (tolerant)
Treatment protocol Treatment with ch128.1Av
2x10e5 IM-9 and cell line: U266 (tolerant) were incubated in 24-well plates under tissue culture conditions (37ºC and 5%CO2) with 10 nM ch128.1Av or buffer for 1h, 3h, 9h, and 24h
Growth protocol Cells were grown in 24-well plates under tissue culture conditions (37ºC and 5%CO2)
Extracted molecule total RNA
Extraction protocol RNA isolation as previously described (Pattenden et al., EMBO (2002) 21: 1978). Targets were prepared from 200 ng total RNA using Illumina. TotalPrep RNA Amplification Kit (Ambion, catalog #AMIL1791).
Label Biotin
Label protocol Illumina protocol
 
Hybridization protocol Illumina HumanRef-8 V2 BeadChips were hybridized, washed, stained and scanned according to Illumina’s protocol using the updated wash procedure
Scan protocol Processed arrays were read using Illumina BeadReader according to the manufacturer's instructions.
Description Time course experiment
Replicate 1/2
U266AT24R1
Data processing Data was extracted with Illumina BeadStudio software.
 
Submission date Feb 09, 2009
Last update date Feb 09, 2009
Contact name Manuel Penichet
E-mail(s) penichet@mednet.ucla.edu
Phone +1-310-825-1304
Fax +1-310-825-7575
Organization name University of California, Los Angeles
Department Department of Surgery
Lab Division of Surgical Oncology
Street address Box 951782
City Los Angeles
State/province CA
ZIP/Postal code 90095-1782
Country USA
 
Platform ID GPL6255
Series (1)
GSE14754 Mechanism of sensitivity and resistance to treatment with an TfR1 antibody fusion protein in B-cell malignancies

Data table header descriptions
ID_REF
VALUE Log2 normalized signal intensity

Data table
ID_REF VALUE
ILMN_10000 7.590587
ILMN_10001 12.387333
ILMN_10002 7.134335
ILMN_10004 7.321332
ILMN_10005 9.844092
ILMN_10006 7.353233
ILMN_10009 7.402144
ILMN_1001 7.274561
ILMN_10010 7.010078
ILMN_10011 9.208696
ILMN_10012 6.929829
ILMN_10013 7.317332
ILMN_10014 7.342106
ILMN_10016 7.405673
ILMN_1002 6.879277
ILMN_10020 9.099968
ILMN_10021 9.99957
ILMN_10022 7.263076
ILMN_10023 6.850098
ILMN_10024 7.137686

Total number of rows: 20589

Table truncated, full table size 398 Kbytes.




Supplementary data files not provided
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap