|
| Status |
Public on Aug 19, 2020 |
| Title |
Fabp2KO-M-1: Fabp2KO_male_430A_replicate1 |
| Sample type |
RNA |
| |
|
| Source name |
small intestine from Fabp2-deleted C57BL/6J mouse
|
| Organism |
Mus musculus |
| Characteristics |
tissue: small intestine
age: 10-week old
genotype: Fabp2-/- C57BL/6J
|
| Treatment protocol |
No treatment
|
| Growth protocol |
Mice were housed in a sterile and temperature controlled environment and fed a standard lab diet (Purina 5001).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
The small intestine, starting from the base of the stomach and ending just before the cecum, was excised and then flushed with ice-cold saline prior to tissue homogenization. RNA was extracted from homogenates using Trizol (Invitrogen) and assessed for integrity (RIN>7) prior to use in microarray analysis.
|
| Label |
Cy3
|
| Label protocol |
Fluorescently labeled cDNA probes for each RNA pool was prepared following the procedures specified by the manufacturer.
|
| |
|
| Hybridization protocol |
cDNA was hybridized to Affymetrix 430A and 430B chips following the procedures specified by the manufacturer.
|
| Scan protocol |
Signals were acquired using the Affymetrix GeneChip Scanner Model 3000.
|
| Description |
gene expression of the small intestine
|
| Data processing |
The data were processed and analyzed using the R statistical environment and Bioconductor software (www.bioconductor.org) and to log2 scale using the RMA algorithm with background correction
|
| |
|
| Submission date |
Mar 26, 2019 |
| Last update date |
Aug 19, 2020 |
| Contact name |
Luis B Agellon |
| E-mail(s) |
luis.agellon@mcgill.ca
|
| Phone |
1-514-398-7862
|
| Organization name |
McGill University
|
| Department |
School of Human Nutrition
|
| Street address |
21111 Lakeshore Road
|
| City |
Ste-Anne-de-Bellevue |
| State/province |
QC |
| ZIP/Postal code |
H9X 3V9 |
| Country |
Canada |
| |
|
| Platform ID |
GPL339 |
| Series (1) |
| GSE128862 |
Sexually dimorphic characteristics of the small intestinal transcriptome of Fabp-gene ablated mice |
|
