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Sample GSM3708 Query DataSets for GSM3708
Status Public on Jan 21, 2003
Title Eye Discs iso4 lmC31
Sample type RNA
 
Source name Drosophila wandering larvae eye imaginal discs
Organism Drosophila melanogaster
Extracted molecule total RNA
 
Description To analyze gene expression in Drosophila eye primordia (iso4 third instar wandering larvae), the eye part was separated from the antennal part of the eye-antennal disc. One hundred eye imaginal discs were dissected, immediately transferred into 800ul Trizol (GIBCO Life Technologies) and stored at –70°C. RNA extraction was performed according to the manufacturer’s instructions. Quantity and quality of total RNA were determined by capillary electrophoresis on a RNA6000 bioanalyzer (Agilent Technologies). Two successive rounds of cDNA synthesis were applied to prepare 20ug of biotinylated target from one to two micrograms of total RNA (see Michaut et al., 2003).
 
Submission date Jan 20, 2003
Last update date Mar 17, 2006
Contact name Lydia Michaut
E-mail(s) Lydia.Michaut@unibas.ch
Phone +41612672054
Fax +41612672078
Organization name Biozentrum
Department Cell Biology
Street address Klingelbergstrasse 70
City Basel
ZIP/Postal code 4056
Country Switzerland
 
Platform ID GPL72
Series (1)
GSE271 Drosophila Eye Development - Larval Stage

Data table header descriptions
ID_REF
Positive Number of positive probe pairs
Negative Number of negative probe pairs
Pairs Number of probe pairs for a particular probe set
Pairs Used Number of probe pairs per probe set used in the analysis
Pairs InAvg Trimmed probed pairs that excluded probes with extremly intense or weak signal
Pos Fraction Number of positive probe pairs/Number of probe pairs used
Log Avg Hybridization performance of a probe set
Pos/Neg Ratio of positive probe pairs to negative probe pairs in a probe set
VALUE Average difference
ABS_CALL Present (P) Marginal (M) Absent (A)

Data table
ID_REF Positive Negative Pairs Pairs Used Pairs InAvg Pos Fraction Log Avg Pos/Neg VALUE ABS_CALL
AFFX-MurIL2_at 2 4 20 20 19 0.1 -0.25 0.5 -36.4 A
AFFX-MurIL10_at 6 5 20 20 18 0.3 0.74 1.2 12.6 A
AFFX-MurIL4_at 3 2 20 20 19 0.15 1.09 1.5 38.7 A
AFFX-MurFAS_at 3 2 20 20 19 0.15 1.32 1.5 16.8 A
AFFX-BioB-5_at 3 6 20 20 19 0.15 -1.52 0.5 -38.4 A
AFFX-BioB-M_at 2 4 20 20 18 0.1 0.01 0.5 -6.6 A
AFFX-BioB-3_at 4 6 20 20 18 0.2 -0.56 0.7 -90.6 A
AFFX-BioC-5_at 5 4 20 20 18 0.25 0.14 1.3 31.9 A
AFFX-BioC-3_at 3 5 20 20 17 0.15 -1.02 0.6 -19.4 A
AFFX-BioDn-5_at 2 7 20 20 19 0.1 -1.43 0.3 -77.1 A
AFFX-BioDn-3_at 4 5 20 20 18 0.2 0.46 0.8 51.2 A
AFFX-CreX-5_at 0 3 20 20 18 0 -0.12 0 -10.6 A
AFFX-CreX-3_at 5 1 20 20 18 0.25 1.28 5 77.7 A
AFFX-DapX-5_at 4 3 20 20 19 0.2 -1.13 1.3 -21 A
AFFX-DapX-M_at 3 4 20 20 19 0.15 -0.24 0.8 17.5 A
AFFX-DapX-3_at 1 8 20 20 19 0.05 -1.63 0.1 -43.3 A
AFFX-LysX-5_at 3 1 20 20 19 0.15 0.37 3 4 A
AFFX-LysX-M_at 2 6 20 20 19 0.1 -0.81 0.3 -62 A
AFFX-LysX-3_at 2 2 20 20 20 0.1 0.07 1 11.9 A
AFFX-PheX-5_at 2 4 20 20 18 0.1 -0.65 0.5 -22.2 A

Total number of rows: 14010

Table truncated, full table size 603 Kbytes.




Supplementary data files not provided
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