|
| Status |
Public on Apr 07, 2009 |
| Title |
SIRT1 LKO liver_4 pm_rep2 |
| Sample type |
RNA |
| |
|
| Source name |
SIRT1 LKO liver, ad lib, 4:00pm
|
| Organism |
Mus musculus |
| Characteristics |
genotype: C57BL/6
gender: male
age: 10 weeks
tissue: Liver
diet: chow diet
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Qiagen RNA easy mini kit with on-column DNAseI treatment
|
| Label |
Cy3
|
| Label protocol |
Starting with 500ng of total RNA, Cy3 labeled cRNA was produced according to manufacturer’s protocol.
|
| |
|
| Hybridization protocol |
For each sample, 1.65ug of Cy3 labeled cRNAs were fragmented and hybridized for 17 hours in a rotating hybridization oven.
|
| Scan protocol |
Slides were washed and then scanned with an Agilent Scanner.
|
| Description |
each sample contains RNA from two SIRT1 LKO animals
|
| Data processing |
Data were obtained and processed with the Agilent Feature Extraction Software (v9.5). The resulting data were imported into Rosetta Resolver (v7.0) and normalized intensities were exported.
|
| |
|
| Submission date |
Feb 18, 2009 |
| Last update date |
Mar 27, 2009 |
| Contact name |
NIEHS Microarray Core |
| E-mail(s) |
microarray@niehs.nih.gov, liuliw@niehs.nih.gov
|
| Organization name |
NIEHS
|
| Department |
DIR
|
| Lab |
Microarray Core
|
| Street address |
111 T.W. Alexander Drive
|
| City |
RTP |
| State/province |
NC |
| ZIP/Postal code |
27709 |
| Country |
USA |
| |
|
| Platform ID |
GPL7202 |
| Series (1) |
| GSE14921 |
Hepatocyte-specific deletion of SIRT1 alters fatty acid metabolism |
|
