|
| Status |
Public on Feb 19, 2009 |
| Title |
Wild-type macrophages 2h |
| Sample type |
RNA |
| |
|
| Source name |
wild-type macrophages stimulated with LPS for 2 h
|
| Organism |
Mus musculus |
| Characteristics |
strain: wild-type
|
| Treatment protocol |
stimulated with 100 ng/ml LPS for indicated periods
|
| Growth protocol |
peritoneal macrophages were preprared from thioglycollate-injected mice.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared by NuGEN Ovation system according to the manufactuerer's inscrution.
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix GeneChip Mouse Genome 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix Gene CHIP scanner 3000.
|
| Description |
none
|
| Data processing |
The data were analyzed with RMA using the arrayassist program (Stratagene).
|
| |
|
| Submission date |
Feb 18, 2009 |
| Last update date |
Aug 02, 2019 |
| Contact name |
Osamu Takeuchi |
| E-mail(s) |
otake@mfour.med.kyoto-u.ac.jp
|
| Phone |
81-75-753-9500
|
| Organization name |
Kyoto University
|
| Department |
Grad School of Medicine
|
| Lab |
Medical Chemistry
|
| Street address |
Yoshida-konoe-cho, Sakyo-ku,
|
| City |
Kyoto |
| State/province |
Kyoto |
| ZIP/Postal code |
606-8501 |
| Country |
Japan |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE14891 |
Expression data of LPS-stimulated macrophages from wild-type and Zc3h12a-/- mice. |
|
| Relations |
| Reanalyzed by |
GSE119085 |
| Reanalyzed by |
GSE135324 |
