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Sample GSM372431 Query DataSets for GSM372431
Status Public on May 05, 2009
Title Atopic subject 26, HDM stimulated CD4 T-cells
Sample type RNA
 
Source name PBMC-derived HDM-stimulated CD4 T-cells at 24 h
Organism Homo sapiens
Characteristics time point: 24 h
Treatment protocol PBMC were cultured in the presence or absence of HDM extract (CSL).
Growth protocol PBMC from cryopreserved stocks were cultured in AIM-V medium for 24 hours.
Extracted molecule total RNA
Extraction protocol At the termination of the cultures, CD4 T-cell were isolated by immunomagnetic separation (DyNAL), and total RNA was extracted by TRIzol (Invitrogen) followed by RNeasy (QIAgen).
Label biotin
Label protocol Total RNA was labeled employing the Whole Transcript (WT) Sense Target Labeling Assay according to the manufacturer’s instructions (Affymetrix).
 
Hybridization protocol The samples were hybridized to HuGene ST1.0 microarrays according to standardized protocols from Affymetrix.
Scan protocol The samples were scanned on a GeneChip® Scanner 3000 7G (Affymetrix).
Description PBMC were from cryopreserved stocks.
Data processing The microarray data were preprocessed in Expression Consol software (Affymetrix) employing the probe logarithmic intensity error algorithm (PLIER, parameters - PM-GCBG background subtraction, quantile normalization, iterPLIER summarization).
 
Submission date Feb 19, 2009
Last update date Sep 01, 2016
Contact name Anthony Bosco
E-mail(s) Anthony.Bosco@telethonkids.org.au
Organization name Telethon Kids Institute
Street address 100 Roberts Road
City Subiaco
State/province WA
ZIP/Postal code 6008
Country Australia
 
Platform ID GPL6244
Series (1)
GSE14908 Global expression profiling of CD4 T-cell responses to house dust mite allergens in human atopics and nonatopics.
Relations
Reanalyzed by GSE86357

Data table header descriptions
ID_REF
VALUE Signal on the log2 scale calculated by Expression Consol software.

Data table
ID_REF VALUE
7896736 4.690400668
7896738 4.179633658
7896740 4.658250106
7896746 7.797012329
7896748 10.08252298
7896750 7.750968875
7896752 9.448298257
7896754 7.255830212
7896756 5.757384142
7896759 6.001854249
7896761 8.292587598
7896779 8.017356296
7896798 7.862641077
7896817 7.386494546
7896822 6.752050219
7896859 6.216117628
7896861 4.703219775
7896863 5.213891981
7896865 6.591632896
7896878 7.435686927

Total number of rows: 33297

Table truncated, full table size 646 Kbytes.




Supplementary file Size Download File type/resource
GSM372431.CEL.gz 3.8 Mb (ftp)(http) CEL
Processed data included within Sample table

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