NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM372462 Query DataSets for GSM372462
Status Public on May 05, 2009
Title Nonatopic subject 12, resting CD4 T-cells
Sample type RNA
 
Source name PBMC-derived unstimulated CD4 T-cells at 24 h
Organism Homo sapiens
Characteristics time point: 24 h
Treatment protocol PBMC were cultured in the presence or absence of HDM extract (CSL).
Growth protocol PBMC from cryopreserved stocks were cultured in AIM-V medium for 24 hours.
Extracted molecule total RNA
Extraction protocol At the termination of the cultures, CD4 T-cell were isolated by immunomagnetic separation (DyNAL), and total RNA was extracted by TRIzol (Invitrogen) followed by RNeasy (QIAgen).
Label biotin
Label protocol Total RNA was labeled employing the Whole Transcript (WT) Sense Target Labeling Assay according to the manufacturer’s instructions (Affymetrix).
 
Hybridization protocol The samples were hybridized to HuGene ST1.0 microarrays according to standardized protocols from Affymetrix.
Scan protocol The samples were scanned on a GeneChip® Scanner 3000 7G (Affymetrix).
Description PBMC were from cryopreserved stocks.
Data processing The microarray data were preprocessed in Expression Consol software (Affymetrix) employing the probe logarithmic intensity error algorithm (PLIER, parameters - PM-GCBG background subtraction, quantile normalization, iterPLIER summarization).
 
Submission date Feb 19, 2009
Last update date Sep 01, 2016
Contact name Anthony Bosco
E-mail(s) Anthony.Bosco@telethonkids.org.au
Organization name Telethon Kids Institute
Street address 100 Roberts Road
City Subiaco
State/province WA
ZIP/Postal code 6008
Country Australia
 
Platform ID GPL6244
Series (1)
GSE14908 Global expression profiling of CD4 T-cell responses to house dust mite allergens in human atopics and nonatopics.
Relations
Reanalyzed by GSE86357

Data table header descriptions
ID_REF
VALUE Signal on the log2 scale calculated by Expression Consol software.

Data table
ID_REF VALUE
7896736 4.989186703
7896738 4.319787424
7896740 4.186323775
7896746 8.147362671
7896748 10.14943594
7896750 7.008057676
7896752 9.402574887
7896754 7.263493628
7896756 6.006564857
7896759 6.23223959
7896761 8.532133416
7896779 8.084415645
7896798 8.078329098
7896817 6.679268817
7896822 6.606791311
7896859 6.316982002
7896861 4.882952584
7896863 5.376257386
7896865 6.936374108
7896878 7.514776376

Total number of rows: 33297

Table truncated, full table size 646 Kbytes.




Supplementary file Size Download File type/resource
GSM372462.CEL.gz 4.0 Mb (ftp)(http) CEL
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap