|
| Status |
Public on Apr 15, 2009 |
| Title |
H322 |
| Sample type |
RNA |
| |
|
| Source name |
human NSCLC cell line H322
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: H322
|
| Growth protocol |
exponentially growing cells
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on U133A. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
gene expression from the human NSCLC cell line H322
|
| Data processing |
Data were processed with dCHIP 1.3.1
|
| |
|
| Submission date |
Feb 20, 2009 |
| Last update date |
Sep 01, 2016 |
| Contact name |
Thomas Zander |
| Organization name |
University Hospital Cologne
|
| Street address |
Kerpenerstr. 62
|
| City |
Cologne |
| ZIP/Postal code |
50924 |
| Country |
Germany |
| |
|
| Platform ID |
GPL96 |
| Series (1) |
| GSE14925 |
Expression data from Non small cell lung cancer cell lines |
|
| Relations |
| Reanalyzed by |
GSE86363 |
