|
| Status |
Public on May 03, 2019 |
| Title |
Saline_3 |
| Sample type |
RNA |
| |
|
| Source name |
Peritoneal tissue
|
| Organism |
Mus musculus |
| Characteristics |
tissue: Peritoneal tissue
treatment: Saline for 6 weeks
|
| Treatment protocol |
8-10 weeks old male C57BL/6 mice were daily intraperitoneally injected with 4.25% peritoneal dialysis fluid (DIANEAL containing 4.25% glucose, Baxter Healthcare) at 0.1 ml/g body weight for 6 weeks. Mice in control group were treated with 0.9% saline at 0.1 ml/g body weight for 6 weeks.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using TRIzol (Invitrogen) and miRNeasy mini kit (QIAGEN) according to manufacturer’s instructions, which efficiently recovered all RNA species, including miRNAs. RNA quality and quantity was measured by using nanodrop spectrophotometer (ND-1000, Nanodrop Technologies) and RNA Integrity was determined by gel electrophoresis.
|
| Label |
Hy3/Hy5
|
| Label protocol |
After RNA isolation from the samples, the miRCURY™ Hy3™/Hy5™ Power labeling kit (Exiqon, Vedbaek, Denmark) was used according to the manufacturer’s guideline for miRNA labelling.
|
| |
|
| Hybridization protocol |
After stopping the labeling procedure, the Hy3TM-labeled samples were hybridized on the miRCURYTM LNA Array (v.16.0) (Exiqon) according to array manual.
|
| Scan protocol |
The slides were scanned using the Axon GenePix 4000B microarray scanner (Axon Instruments, Foster City, CA).
|
| Description |
Total RNA
|
| Data processing |
Scanned images were then imported into GenePix Pro 6.0 software (Axon) for grid alignment and data extraction. Replicated miRNAs were averaged and miRNAs that intensities>=30 in all samples were chosen for calculating normalization factor.
|
| |
|
| Submission date |
Apr 26, 2019 |
| Last update date |
May 04, 2019 |
| Contact name |
Meijun Si |
| E-mail(s) |
simeijunsysu@163.com
|
| Organization name |
The 3rd Affiliated Hospital of Sun Yat-sen University
|
| Department |
Nephrology
|
| Street address |
600 Tianhe Ave.
|
| City |
Guangzhou |
| State/province |
Guangdong |
| ZIP/Postal code |
510630 |
| Country |
China |
| |
|
| Platform ID |
GPL17107 |
| Series (1) |
| GSE130387 |
microRNA in mouse peritoneal tissue |
|
