|
| Status |
Public on Mar 01, 2010 |
| Title |
Pool13 |
| Sample type |
RNA |
| |
|
| Source name |
hypothalamus
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6J
genotype: wild-type
age: P13
fasting: No
pool: 13
sample id: GW
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocols.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
replicate 1
|
| Data processing |
The data were normalised using quantile normalisation in R
|
| |
|
| Submission date |
Feb 25, 2009 |
| Last update date |
Mar 03, 2011 |
| Contact name |
Jun Li |
| E-mail(s) |
junzli@umich.edu
|
| Phone |
734-615-5754
|
| Organization name |
University of Michigan
|
| Street address |
5789A Medical Science II, Box 5618
|
| City |
Ann Arbor |
| State/province |
MI |
| ZIP/Postal code |
48109 |
| Country |
USA |
| |
|
| Platform ID |
GPL6103 |
| Series (1) |
| GSE14984 |
Neonatal starvation response and developmental changes in gene expression revealed by hypothalamic expression profiling |
|
