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Sample GSM374324 Query DataSets for GSM374324
Status Public on Feb 26, 2009
Title 10h mosquito embryo RNA, biological replicate 1
Sample type RNA
 
Source name 10h mosquito embryo
Organism Anopheles gambiae
Characteristics strain: Kisumu
Treatment protocol Staged mosquito eggs were collected into meshed basket, treated in 25% household bleach for 1min.16 seconds, washed thoroughly with streaming water and subjected to RNA prep.
Growth protocol Mosquito eggs were aged in water at 25C.
Extracted molecule total RNA
Extraction protocol Total RNA extraction were performed by Trizol, followed by purification on RNAeasy (Qiagen) column.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix one-step protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Regular, following the guidelines from Affymetrix.
Scan protocol Arrays were hybridized and scanned with a GeneArray Scanner (Hewlett-Packard/Affymetrix).
Description for further information please refer to (Goltsev et al, Developmental and evolutionary basis for drought tolerance of the Anopheles gambiae embryo. 2009, Developmental Biology)
Data processing RMA-express software was used for processing of raw expression data and estimation of log base 2 expression values.
 
Submission date Feb 25, 2009
Last update date Feb 25, 2009
Contact name Yury Goltsev
E-mail(s) goltsev@berkeley.edu, mlevine@berkeley.edu
Phone 510 642 5007
Organization name UC Berkeley
Department MCB
Lab Levine
Street address 585 LSA, UC Berkeley
City Berkeley
State/province CA
ZIP/Postal code 94720
Country USA
 
Platform ID GPL1321
Series (2)
GSE14993 Developmental time course of gene expression in Anopheles gambiae embryo
GSE15001 Gene expression in the Anopheles gambiae embryo

Data table header descriptions
ID_REF
VALUE log2 of signal intensity (RMA)

Data table
ID_REF VALUE
AF002237.1_at 4.247566
AF203338.1_s_at 4.936862
AF203339.1_at 8.470157
AF203339.1_s_at 8.535761
AF283273.1_s_at 6.352798
AF298053.1_at 3.389632
AJ000037.1_s_at 5.236453
AJ130951.1_RC_at 7.592149
AJ130951.1_RC_s_at 5.152837
AJ130951.1_at 3.500677
AJ297931.1_at 4.140755
AJ297931.1_s_at 5.337139
AJ302654.1_RC_at 5.738331
AJ302654.1_at 3.939937
AJ302661.1_at 4.807132
AJ302661.1_s_at 6.551034
AJ304410.1_s_at 6.523453
AY039510.1_at 4.276628
AY062189.1_s_at 4.312916
AY062190.1_s_at 7.978724

Total number of rows: 16167

Table truncated, full table size 451 Kbytes.




Supplementary file Size Download File type/resource
GSM374324.CEL.gz 2.0 Mb (ftp)(http) CEL
Processed data included within Sample table

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