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Status
Public on Mar 02, 2005
Title
HEK293T cells, Forskolin 2
Sample type
RNA
Source name
Human HEK293T cell line
Organism
Homo sapiens
Extracted molecule
total RNA
Description
Culture of human HEK293T cells, with 1hr forskolin (a cAMP agonist) treatment. RNA was extracted using the Qiagen RNeasy kit. Targets were produced using standard Affymetrix procedures from about 5ug of total RNA. 2 control and 2 forskolin treated samples, and 2 forskolin treated samples from ACREB transfected cells were analyzed. Data was analyzed using dChIP software (www.dchip.org). Keywords = HEK293T, cAMP induced genes, forskolin, CREB target genes
Submission date
Dec 10, 2004
Last update date
May 27, 2005
Contact name
Xinmin Zhang
E-mail(s)
xzhang@salk.edu
Phone
858-453-4100 x1157
Organization name
Salk Institute
Street address
10010 N. Torrey Pines Rd.
City
La Jolla
State/province
CA
ZIP/Postal code
92037
Country
USA
Platform ID
GPL96
Series (1)
GSE2060
Characterize CREB target genes in different tissue types
Data table header descriptions
ID_REF
VALUE
'expression index' a measure of the abundance of a transcript, determined using the dChip software with PM-MM models.
Data table
ID_REF
VALUE
AFFX-BioB-5_at
431.291
AFFX-BioB-M_at
479.121
AFFX-BioB-3_at
477.881
AFFX-BioC-5_at
641.258
AFFX-BioC-3_at
757.971
AFFX-BioDn-5_at
808.302
AFFX-BioDn-3_at
2398.78
AFFX-CreX-5_at
6221.08
AFFX-CreX-3_at
6986.11
AFFX-DapX-5_at
34.4391
AFFX-DapX-M_at
41.1269
AFFX-DapX-3_at
103.931
AFFX-LysX-5_at
-4.20584
AFFX-LysX-M_at
118.21
AFFX-LysX-3_at
64.9958
AFFX-PheX-5_at
-29.5018
AFFX-PheX-M_at
283.221
AFFX-PheX-3_at
69.3306
AFFX-ThrX-5_at
98.6995
AFFX-ThrX-M_at
27.1356
Total number of rows: 22283 Table truncated, full table size 410 Kbytes .Supplementary file
Size
Download
File type/resource
GSM37488.CEL.gz
3.4 Mb
(ftp) (http)
CEL
GSM37488.EXP.gz
344 b
(ftp) (http)
EXP