Culture of human HEK293T cells, transfected with ACREB, a dominant negative form of the CREB protein, and treated with 1hr forskolin (a cAMP agonist). RNA was extracted using the Qiagen RNeasy kit. Targets were produced using standard Affymetrix procedures from about 5ug of total RNA. 2 control and 2 forskolin treated samples, and 2 forskolin treated samples from ACREB transfected cells were analyzed. Data was analyzed using dChIP software (www.dchip.org). Keywords = HEK293T, cAMP induced genes, forskolin, CREB target genes
