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Sample GSM37621 Query DataSets for GSM37621
Status Public on Dec 17, 2004
Title CENPK113.7D N-lim1
Sample type RNA
 
Source name chemostat sampled cells
Organism Saccharomyces cerevisiae
Extracted molecule total RNA
 
Description The S. cerevisiae strains used in this study was the haploid, prototrophic strain CEN.PK113-7D (MATa LEU3). Strains were grown at 30 °C in 2-liter chemostats (Applikon), with a working volume of 1.0 liter as described in van den Berg et al.. Cultures were fed with defined synthetic media based on the medium described by Verduyn et al . For nitrogen-limited growth the medium contained the following: (NH4)2SO4, 1.0 g·l-1; K2SO4, 5.3 g·l-1; KH2PO4, 3.0 g·l-1; MgSO4·7H2O, 0.5 g·l-1; vitamins and trace elements. Glucose concentrations in the feed were designed to obtain approximately 17 g·l-1 residual glucose in the culture broth and thus to avoid transcriptional responses as a result of different degrees of glucose repression. Feed concentrations were 59 g·l-1 glucose (reference strain) and 53 g·l-1 glucose (leu3D strain). The dilution rate was set at 0.10 h-1. The pH was measured online and kept constant at 5.0 by the automatic addition of 2 M KOH with the use of an Applikon ADI 1030 biocontroller. Stirrer speed was 800 rpm and the airflow was 0.5 l.min-1. Dissolved oxygen tension was measured online with an Ingold model 34 100 3002 probe, and was above 50% air saturation. A condenser connected to a cryostat set at 2 °C cooled the off-gas, and oxygen and carbon dioxide were measured off-line. Steady-state samples were taken between 10 and 14 volume changes to avoid strain adaptation due to long term cultivation. Dry weight, metabolite-, and gas profiles were constant over at least 3 volume changes prior to sampling for RNA extraction
 
Submission date Dec 15, 2004
Last update date Oct 28, 2005
Contact name Jean-Marc Daran
E-mail(s) j.g.daran@tudelft.nl
Phone +31 15 278 2412
Organization name Delft University of Technology
Department Department of Biotechnology
Lab Kluyver centre for genomics of industrial organisms
Street address Julianalaan 67
City Delft
ZIP/Postal code 2628BC
Country Netherlands
 
Platform ID GPL90
Series (1)
GSE2076 leu3p dependent transcription

Data table header descriptions
ID_REF
VALUE hybridisation signal
ABS_CALL detection call
DETECTION P-VALUE detection significance

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
10000_at 2.1 A 0.418069
10001_at 84.1 P 0.000219
10002_i_at 500.8 A 0.25
10003_f_at 1716.3 P 0.000219
10004_at 0.6 A 0.94553
10005_at 153.2 P 0.000219
10006_at 55.6 P 0.003062
10007_at 193.8 P 0.000322
10008_at 0.5 A 0.901946
10009_at 6.9 A 0.107301
10010_at 897.5 P 0.000219
10011_at 3.4 A 0.267463
10012_at 82.6 P 0.000219
10013_at 90.7 P 0.000219
10014_at 536.5 P 0.000219
10015_at 774.7 P 0.000219
10016_at 324.5 P 0.000219
10017_at 0.7 A 0.810313
10018_at 2850.5 P 0.000219
10019_at 1224.3 P 0.000219

Total number of rows: 9335

Table truncated, full table size 222 Kbytes.




Supplementary data files not provided

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