|
Status |
Public on Mar 07, 2009 |
Title |
Golden Retriever, hemangiosarcoma: ChadG4 |
Sample type |
RNA |
|
|
Source name |
primary hemangiosarcoma tumor cell line
|
Organism |
Canis lupus familiaris |
Characteristics |
tumor type and origin: hemangiosarcoma, spleen breed: Golden Retriever
|
Growth protocol |
Cryopreserved cultured cells from the earliest available passages were used
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA was prepared using the RNEasy Mini Kit and QIAshredder columns (QIAGEN, Valencia, CA)
|
Label |
Biotin
|
Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2.5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
|
|
Hybridization protocol |
Following fragmentation, 20 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Canine 2 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
|
Description |
Gene expression data from dogs with hemangiosarcoma of different breeds
|
Data processing |
The data were background corrected with the RMA algorithm using R and the Bioconductor's affy package. All parameters were set at its default settings
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|
|
Submission date |
Mar 03, 2009 |
Last update date |
Mar 06, 2009 |
Contact name |
Beth Tamburini |
E-mail(s) |
beth.tamburini@ucdenver.edu
|
Organization name |
University of Colorado Denver
|
Department |
Immunology
|
Street address |
1400 Jackson Street, K503
|
City |
Denver |
State/province |
CO |
ZIP/Postal code |
80206 |
Country |
USA |
|
|
Platform ID |
GPL3738 |
Series (2) |
GSE15086 |
Gene Expression Profiles of Sporadic Canine Hemangiosarcoma are Uniquely Associated with Breed |
GSE23760 |
Gene Expression Profiling Identifies Inflammation and Angiogenesis as Distinguishing Features of Canine Hemangiosarcoma |
|