|
| Status |
Public on Mar 07, 2009 |
| Title |
Dalmation, hemangiosarcoma: Dal4 |
| Sample type |
RNA |
| |
|
| Source name |
primary hemangiosarcoma tumor cell line
|
| Organism |
Canis lupus familiaris |
| Characteristics |
tumor type and origin: hemangiosarcoma, spleen
breed: Dalmation
|
| Growth protocol |
Cryopreserved cultured cells from the earliest available passages were used
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was prepared using the RNEasy Mini Kit and QIAshredder columns (QIAGEN, Valencia, CA)
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2.5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 20 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Canine 2 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
|
| Description |
Gene expression data from dogs with hemangiosarcoma of different breeds
|
| Data processing |
The data were background corrected with the RMA algorithm using R and the Bioconductor's affy package. All parameters were set at its default settings
|
| |
|
| Submission date |
Mar 03, 2009 |
| Last update date |
Mar 06, 2009 |
| Contact name |
Beth Tamburini |
| E-mail(s) |
beth.tamburini@ucdenver.edu
|
| Organization name |
University of Colorado Denver
|
| Department |
Immunology
|
| Street address |
1400 Jackson Street, K503
|
| City |
Denver |
| State/province |
CO |
| ZIP/Postal code |
80206 |
| Country |
USA |
| |
|
| Platform ID |
GPL3738 |
| Series (2) |
| GSE15086 |
Gene Expression Profiles of Sporadic Canine Hemangiosarcoma are Uniquely Associated with Breed |
| GSE23760 |
Gene Expression Profiling Identifies Inflammation and Angiogenesis as Distinguishing Features of Canine Hemangiosarcoma |
|
