|
Status |
Public on May 16, 2022 |
Title |
hMonocyte_Day0_Donor4 |
Sample type |
SRA |
|
|
Source name |
Peripheral blood monocytes
|
Organism |
Homo sapiens |
Characteristics |
treatment: none differentiation conditions: none
|
Treatment protocol |
Differentiation into Mo-M was done in the presence of 10μM AMTB or 0.01% DMSO (vehicle control)
|
Growth protocol |
CD14+ monocytes were isolated from the peripheral blood of healthy volunteers and differentiated into macrophages (Mo-M) in the presence of 100ng/ml M-CSF
|
Extracted molecule |
polyA RNA |
Extraction protocol |
RNA was isolated from CD14+ monocyte, Mo-M-DMSO and Mo-M-AMTB samples using an RNAeasy Mini kit PLUS (Qiagen) RNA libraries were prepared for sequencing using NEBNext Ultra II Directional RNA Library Prep Kit (NEBL)
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina NextSeq 500 |
|
|
Data processing |
Transcript counts were then generated using Salmon against the GRCh38 transcriptome (Ensembl release 92). Tximport was used to collapse multiple transcripts per gene into a single expression estimate for each protein-coding gene. Differential expression analysis was performed using DESeq2 with default settings. Genome_build: GRCh38 Supplementary_files_format_and_content: DESeq2 results are provided (at gene level) in addition to individual transript abundance files from salmon.
|
|
|
Submission date |
May 17, 2019 |
Last update date |
May 17, 2022 |
Contact name |
Hamish W King |
Organization name |
Queen Mary University of London
|
Department |
Blizard Institute
|
Street address |
4 Newark St
|
City |
London |
ZIP/Postal code |
E1 2AT |
Country |
United Kingdom |
|
|
Platform ID |
GPL18573 |
Series (1) |
GSE131415 |
Constitutive activity of the cation channel TRPM8 regulates the function and differentiation of human monocytes |
|
Relations |
BioSample |
SAMN11671048 |
SRA |
SRX5857328 |