|
| Status |
Public on Jun 01, 2009 |
| Title |
HSF1.ESC |
| Sample type |
RNA |
| |
|
| Source name |
HSF1, ESC
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: HSF1
stage: ESC
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with Trizol reagent and alcohol precipitated. Quality control was performed with Agilent Bioanalyser.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays.
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol.
|
| Scan protocol |
Standard Illumina scanning protocol.
|
| Description |
ESC stage from HSF1 cultures.
|
| Data processing |
The data were normalised using quantile normalisation with BeadStudio.
|
| |
|
| Submission date |
Mar 12, 2009 |
| Last update date |
Mar 12, 2009 |
| Contact name |
Ronald P. Hart |
| E-mail(s) |
rhart@rutgers.edu
|
| Phone |
848-445-1783
|
| Organization name |
Rutgers University
|
| Department |
Cell Biology & Neuroscience
|
| Street address |
604 Allison Rd Rm B430
|
| City |
Piscataway |
| State/province |
NJ |
| ZIP/Postal code |
08854 |
| Country |
USA |
| |
|
| Platform ID |
GPL6884 |
| Series (1) |
| GSE15206 |
Identification of differentiation status of human embryonic stem cell cultures. |
|
