|
| Status |
Public on Mar 14, 2009 |
| Title |
T+L6h rep1 |
| Sample type |
RNA |
| |
|
| Source name |
Endotoxin-tolerant human monocytes re-stimulated with LPS 6h
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: Human monocyte
|
| Growth protocol |
For microarray transcriptome profiling of LPS tolerized human monocytes restimulated with LPS for 3, 6 and 24h. Human monocytes were isolated from buffy coats and cultured in DMEM media containing antibiotics (100IU/ml penicillin and 100ug/ml streptomycin; Invitrogen) for 2h. Non-adherant cells were removed by washing and cells were thereafter cultured in DMEM media containing antibiotics (100IU/ml penicillin and 100ug/ml streptomycin; Invitrogen) and 10% heat inactivated fetal calf serum (Pasching, Austria). These cells were used for all subsequent treatments described above.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using Trizol (Invitrogen) and column-purified with RNeasy kits (Qiagen).
|
| Label |
biotin
|
| Label protocol |
According to Illumina Protocol
|
| |
|
| Hybridization protocol |
According to Illumina Protocol
|
| Scan protocol |
Illumina BeadScan
|
| Description |
Human monocyte exposure to LPS 8h. Wash and leaving at incubator for 16 h. After that, the cells were re-stimulated with 10 ng/ml LPS 6h
|
| Data processing |
Data were normalized with cross-correlation method and differential expression was based on the mean of the log2 fold changes.
|
| |
|
| Submission date |
Mar 13, 2009 |
| Last update date |
Mar 13, 2009 |
| Contact name |
Eduardo Lopez-Collazo |
| E-mail(s) |
elopezc.hulp@salud.madrid.org
|
| Phone |
0034912071029
|
| Organization name |
Research Unit, Hospital La Paz
|
| Lab |
Tumorinmunology
|
| Street address |
Paseo de la Castellana, 261
|
| City |
Madrid |
| ZIP/Postal code |
28046 |
| Country |
Spain |
| |
|
| Platform ID |
GPL6255 |
| Series (1) |
| GSE15219 |
Transcriptome analysis of in vitro endotoxin tolerant human monocytes |
|
