|
| Status |
Public on Mar 24, 2009 |
| Title |
Disc tissue 3 |
| Sample type |
RNA |
| |
|
| Source name |
Disc Tissue
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: Disc Tissue
site: L4-L5
grade: III
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Cells were collected via laser capture mircodissection (LCM) and total RNA was isolated according to the Paradise reagent system.
|
| Label |
biotin
|
| Label protocol |
Biotin-labeled cRNA were prepard according to the Affymetrix protocol for IVT labeling.
|
| |
|
| Hybridization protocol |
Fragmented cRNA was hybridized to the X3P chip in the Affymetrix hybridiztion buffer for 16 hrs at 45oC. X3P chips were washed and labeled in the Affymetrix Fluidics Station.
|
| Scan protocol |
GeneChips were scanned once via the Affymetrix 3000G scanner.
|
| Description |
Surgical specimen
|
| Data processing |
The data were analyzed with the GCOS Affymetrix GeneChip Operating System (version 1.2) and Microarray Suite version 5.0 (MAS 5.0). Affymetrix default analysis settings and global scaling as normalization were used.
|
| |
|
| Submission date |
Mar 13, 2009 |
| Last update date |
Mar 24, 2009 |
| Contact name |
Helen Gruber |
| E-mail(s) |
Helen.Gruber@carolinashealthcare.org
|
| Organization name |
Carolinas HealthCare System
|
| Department |
Orthopaedic Surgery
|
| Lab |
Orthopaedic Research
|
| Street address |
1542 Graden Terrace
|
| City |
Charlotte |
| State/province |
NC |
| ZIP/Postal code |
28203 |
| Country |
USA |
| |
|
| Platform ID |
GPL1352 |
| Series (1) |
| GSE15227 |
Asporin is Expressed at Higher Levels in the More Degenerate Human Intervertebral Disc Tissue |
|
