|
| Status |
Public on Aug 31, 2010 |
| Title |
Biliary Atresia Patient 30 |
| Sample type |
RNA |
| |
|
| Source name |
Human Liver Wedge Biopsy
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: Liver Wedge Biopsy
molecular group: Inflammation
|
| Treatment protocol |
Flash Frozen
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNeasy Mini Kit (QIAGEN, California, USA)
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 400 ng total RNA amplified with TargetAmp1-Round Aminoallyl-aRNA Amplification Kit 101 (Epicentre, WI) using both the TargetAmp t7-Oligo (dT) Primer A (Epicentre, WI) and the Full Spectrum MultiStart Primers for T7 IVT (10X) (SBI System Biosciences, CA) along with SuperScript III Reverse Transcriptase (Invitrogen, CA).
|
| |
|
| Hybridization protocol |
Following chemical fragmentation of biotinylated-labeled cRNA, 10ug of cRNA were hybridized for 16hr at 45 degC on the human HG-U133 Plus 2.0 GeneChip array. Gene Chips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000
|
| Description |
Gene expression data from human liver.
|
| Data processing |
The data was analyzed with GeneSpring version 7.3 using RMA normalization, then normalized to the median of all samples.
|
| |
|
| Submission date |
Mar 15, 2009 |
| Last update date |
Aug 31, 2010 |
| Contact name |
Jorge A Bezerra |
| E-mail(s) |
jorge.bezerra@cchmc.org
|
| Organization name |
Cincinnati Children's Hospital Medical Center
|
| Department |
Gastroenterology, Hepatology & Nutrition
|
| Street address |
3333 Burnet Ave.
|
| City |
Cincinnati |
| State/province |
OH |
| ZIP/Postal code |
45229 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE15235 |
Staging of biliary atresia at diagnosis by molecular profiling of the liver |
|
