|
| Status |
Public on Jun 01, 2009 |
| Title |
LLC-Moil-24h-rep2, rank invariant |
| Sample type |
RNA |
| |
|
| Source name |
Lewis lung carcinoma cells, Mastic Oil, 24h treatment
|
| Organism |
Mus musculus |
| Characteristics |
cell line: Lung adenocarcinoma (LLC)
treatment time: 24h
strain: C57BL
|
| Treatment protocol |
Cells were treated with 0.01% v/v mastic oil or 0.1% DMSO vehicle for the indicated periods of time (3 to 48h).
|
| Growth protocol |
Cells were cultured in DMEM supplemented with 10% FCS L-glutamine and antibiotics (Gibco, Grand Island, NY).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated with the Trizol Plus kit (Invitrogen) according to manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA was prepared with Illumina TotalPrep RNA Amplification Kit.
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol.
|
| Scan protocol |
Standard Illumina scanning protocol.
|
| Description |
Replicate 2 of 2.
Mastic oil 24h - 2
|
| Data processing |
Data were normalized with rank invariant normalization, and statistical analysis was perfomed using ANOVA and clustering analysis with k-means clustering.
|
| |
|
| Submission date |
Mar 18, 2009 |
| Last update date |
Mar 18, 2009 |
| Contact name |
Fragiskos N Kolisis |
| E-mail(s) |
kolisis@eie.gr
|
| Phone |
+30 210 7273759
|
| Fax |
+30 210 7273758
|
| URL |
http://www.eie.gr/nhrf/institutes/ibrb/programmes/metabolicengineering-en.html
|
| Organization name |
National Hellenic Research Foundation
|
| Department |
Institute of Biological Research and Biotechnology
|
| Street address |
48 Vasileos Constantinou ave.
|
| City |
Athens |
| ZIP/Postal code |
11635 |
| Country |
Greece |
| |
|
| Platform ID |
GPL6333 |
| Series (1) |
| GSE15287 |
Transcriptomic computational analysis of mastic oil-treated Lewis lung carcinomas |
|
