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Status |
Public on Jun 01, 2009 |
Title |
LLC-DMSO-24h-rep1, quantile |
Sample type |
RNA |
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Source name |
Lewis lung carcinoma cells, DMSO, 24h treatment
|
Organism |
Mus musculus |
Characteristics |
cell line: Lung adenocarcinoma (LLC) treatment time: 24h strain: C57BL
|
Treatment protocol |
Cells were treated with 0.01% v/v mastic oil or 0.1% DMSO vehicle for the indicated periods of time (3 to 48h).
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Growth protocol |
Cells were cultured in DMEM supplemented with 10% FCS L-glutamine and antibiotics (Gibco, Grand Island, NY).
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was isolated with the Trizol Plus kit (Invitrogen) according to manufacturer's instructions.
|
Label |
biotin
|
Label protocol |
Biotinylated cRNA was prepared with Illumina TotalPrep RNA Amplification Kit.
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|
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Hybridization protocol |
Standard Illumina hybridization protocol.
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Scan protocol |
Standard Illumina scanning protocol.
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Description |
Replicate 1 of 2. Vehicle 24h - 1
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Data processing |
Data were normalized with quantile normalization, and statistical analysis was perfomed using ANOVA and clustering analysis with k-means clustering. Prior to quantile normalization, probesets absent to all conditions and replicates were removed.
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Submission date |
Mar 18, 2009 |
Last update date |
Mar 18, 2009 |
Contact name |
Fragiskos N Kolisis |
E-mail(s) |
kolisis@eie.gr
|
Phone |
+30 210 7273759
|
Fax |
+30 210 7273758
|
URL |
http://www.eie.gr/nhrf/institutes/ibrb/programmes/metabolicengineering-en.html
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Organization name |
National Hellenic Research Foundation
|
Department |
Institute of Biological Research and Biotechnology
|
Street address |
48 Vasileos Constantinou ave.
|
City |
Athens |
ZIP/Postal code |
11635 |
Country |
Greece |
|
|
Platform ID |
GPL6333 |
Series (1) |
GSE15287 |
Transcriptomic computational analysis of mastic oil-treated Lewis lung carcinomas |
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