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Sample GSM381801 Query DataSets for GSM381801
Status Public on Jun 01, 2009
Title LLC-DMSO-24h-rep1, quantile
Sample type RNA
 
Source name Lewis lung carcinoma cells, DMSO, 24h treatment
Organism Mus musculus
Characteristics cell line: Lung adenocarcinoma (LLC)
treatment time: 24h
strain: C57BL
Treatment protocol Cells were treated with 0.01% v/v mastic oil or 0.1% DMSO vehicle for the indicated periods of time (3 to 48h).
Growth protocol Cells were cultured in DMEM supplemented with 10% FCS L-glutamine and antibiotics (Gibco, Grand Island, NY).
Extracted molecule total RNA
Extraction protocol Total RNA was isolated with the Trizol Plus kit (Invitrogen) according to manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA was prepared with Illumina TotalPrep RNA Amplification Kit.
 
Hybridization protocol Standard Illumina hybridization protocol.
Scan protocol Standard Illumina scanning protocol.
Description Replicate 1 of 2.
Vehicle 24h - 1
Data processing Data were normalized with quantile normalization, and statistical analysis was perfomed using ANOVA and clustering analysis with k-means clustering. Prior to quantile normalization, probesets absent to all conditions and replicates were removed.
 
Submission date Mar 18, 2009
Last update date Mar 18, 2009
Contact name Fragiskos N Kolisis
E-mail(s) kolisis@eie.gr
Phone +30 210 7273759
Fax +30 210 7273758
URL http://www.eie.gr/nhrf/institutes/ibrb/programmes/metabolicengineering-en.html
Organization name National Hellenic Research Foundation
Department Institute of Biological Research and Biotechnology
Street address 48 Vasileos Constantinou ave.
City Athens
ZIP/Postal code 11635
Country Greece
 
Platform ID GPL6333
Series (1)
GSE15287 Transcriptomic computational analysis of mastic oil-treated Lewis lung carcinomas

Data table header descriptions
ID_REF
VALUE Average bead signal intensity, quantile normalization

Data table
ID_REF VALUE
18S_rRNA_X00686_301-S 38479.24
18S_rRNA_X00686_523-S 12145.15
18S_rRNA_X00686_849-S 17521.19
28S_rRNA_X00525_656-S 17.62
9626096_327-S 55.10
9626096_7-S 54.38
9626100_15-S 1812.37
9626100_224-S 5069.39
9626100_24-S 152.58
9626123_31_rc-S 30.01
9626953_200-S 1205.76
9626958_317-S 16119.75
9626958_329-S 110.83
9626962_211_rc-S 32.92
9626962_229-S 84.63
9626962_5-S 131.90
9626984_7-S 59.20
9628654_7-S 93.18
9629514_325-S 75.78
9629553_3793-S 33.87

Total number of rows: 16414

Table truncated, full table size 428 Kbytes.




Supplementary data files not provided
Processed data included within Sample table

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