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Sample GSM383854 Query DataSets for GSM383854
Status Public on Mar 20, 2009
Title SAGE_Cartilage_dedifferentiated_chondrosarcoma_metastasis_CL_UIEK0
Sample type SAGE
Anchor NlaIII
Tag Count 37747
Tag Length 10
 
Source name cartilage, dedifferentiated, chondrosarcoma, metastasis, cell line, adult, female, short sage
Organism Homo sapiens
Characteristics organ/tissue: cartilage
tissue preparation: cell line
age: 45 years old
sex: female
cell type: Dedifferentiated chondrosarcoma lung metastasis
mutations: N/A
other information: Tissue section of lung right lower lobe, middle posterior metastatic lesion from 45 years old female with metastatic dedifferentiated chondrosarcoma were used to obtain mono-dispersed cells. The cell line was established from the cells after culturing in RPMI-1640 supplemented with 10% FBS and 0.1% gentamicin sulfate (Gemini Bioproducts). The cell line had gone through 4 doublings to yield 2 x 107 cells. The cell line was routinely screened for mycoplasma sps. Using a PCR-based ELISA detection assay (Roche). Cell manipulations were always performed on 80-90% confluent cultures for consistency. mRNA was isolated using a direct mRNA extraction kit.
Biomaterial provider Hendrix, M. Seftor, E. Dep of Anatomy and Cell Biology, Holden Comprehensive Cancer Center (University of Iowa).
Extracted molecule total RNA
Extraction protocol N/A
 
Description CGAP_Numeric_library_ID: 609
Tagging_enzyme: BsmF I
Library_producer: S. Malchenko
Laboratory: B. Soares Laboratory (University of Iowa), contributed to the National Cancer Institute Genome Anatomy Project
 
Submission date Mar 19, 2009
Last update date Mar 20, 2009
Contact name Cancer Genome Anatomy Project CGAP
E-mail(s) cgapbs-r@mail.nih.gov
Phone 301-496-1550
URL http://cgap.nci.nih.gov/
Organization name National Cancer Institute
Department Cancer Genome Anatomy Project
Street address
City Bethesda
State/province MD
ZIP/Postal code 20852
Country USA
 
Platform ID GPL4
Series (2)
GSE14 Cancer Genome Anatomy Project (CGAP) SAGE Libraries
GSE15309 Cancer Genome Anatomy Project (CGAP) Human SAGE

Data table header descriptions
TAG
COUNT
TPM tags per million

Data table
TAG COUNT TPM
AAAAAAAAAA 8 211.94
AAAAAAAAAC 1 26.49
AAAAAAAATG 1 26.49
AAAAAAACTC 1 26.49
AAAAAAAGCA 1 26.49
AAAAAAATCA 1 26.49
AAAAAACATT 1 26.49
AAAAAACCCA 1 26.49
AAAAAACTCA 1 26.49
AAAAAACTCC 4 105.97
AAAAAAGCAG 1 26.49
AAAAAATAAA 2 52.98
AAAAAATATG 1 26.49
AAAAAATGAT 1 26.49
AAAAAATTTG 1 26.49
AAAAACACGG 1 26.49
AAAAACATTA 1 26.49
AAAAACATTC 15 397.38
AAAAACCAGT 1 26.49
AAAAACCATA 2 52.98

Total number of rows: 18156

Table truncated, full table size 338 Kbytes.




Supplementary data files not provided

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