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Sample GSM383956 Query DataSets for GSM383956
Status Public on Mar 20, 2009
Title SAGE_Prostate_carcinoma_CL_LNCaP-T
Sample type SAGE
Anchor NlaIII
Tag Count 43542
Tag Length 10
 
Source name prostate, carcinoma, cell line, short SAGE, SAGE, non-normalized
Organism Homo sapiens
Characteristics organ/tissue: prostate
tissue preparation: cell line
cell type: carcinoma
other information: LNCaP cells were maintained in RPMI 1640 supplemented with 10% fetal bovine serum and experiments were performed on cells between passages 20 and 30. For the studies of androgen regulation, charcoal/dextran stripped androgen-free FBS was used. LNCaP cells were cultured first in RPMI 1640 with 10% cFBS for 5 days and then stimulated with an non-metabolizable androgen analog R1881 (DUPONT, Boston, MA) 10-8 M for 24 hours (for generating LNCaP-T library). LNCaP cells identically treated but without R1881 treatment served as control (for generating LNCaP-C library). Cells were harvested at indicated time and polyA+ RNA was double-selected with Fast Track kit (Invitrogene) SAGE libraries were generated according to the procedure provided by Dr. Kinzler (Johns Hopkins University School of Medicine, Baltimore, MD).
Biomaterial provider LNCaP cells (Item Number CRL-1740, American Type Culture Collection, Rockville, MD)
Extracted molecule total RNA
Extraction protocol N/A
 
Description CGAP_Numeric_library_ID: 70
Tagging_enzyme: BsmF I
Old_library_name: SAGE_CPDR_LNCaP-T
Library_producer: L. L. Xu
Laboratory: S. Srivastava, Uniformed Services University of the Health Sciences
References: L. L. Xu et al., Genomics 66, 257-63 (2000) [PUBMED=10873380]|L. L. Xu et al., Int J Cancer 92, 322-8 (2001) [PUBMED=11291065]
 
Submission date Mar 19, 2009
Last update date Mar 20, 2009
Contact name Cancer Genome Anatomy Project CGAP
E-mail(s) cgapbs-r@mail.nih.gov
Phone 301-496-1550
URL http://cgap.nci.nih.gov/
Organization name National Cancer Institute
Department Cancer Genome Anatomy Project
Street address
City Bethesda
State/province MD
ZIP/Postal code 20852
Country USA
 
Platform ID GPL4
Series (2)
GSE14 Cancer Genome Anatomy Project (CGAP) SAGE Libraries
GSE15309 Cancer Genome Anatomy Project (CGAP) Human SAGE

Data table header descriptions
TAG
COUNT
TPM tags per million

Data table
TAG COUNT TPM
AAAAAAAAAA 225 5167.42
AAAAAAAAAC 2 45.93
AAAAAAAAAG 2 45.93
AAAAAAAACA 1 22.97
AAAAAAAAGA 1 22.97
AAAAAAACAG 1 22.97
AAAAAAACTA 1 22.97
AAAAAAATAA 1 22.97
AAAAAAATGA 1 22.97
AAAAAACAGC 1 22.97
AAAAAACCCA 4 91.87
AAAAAACTCC 1 22.97
AAAAAAGAAA 1 22.97
AAAAAAGAAT 1 22.97
AAAAAAGGAC 1 22.97
AAAAAATAAA 2 45.93
AAAAAATTTA 1 22.97
AAAAACAAAT 1 22.97
AAAAACACAA 1 22.97
AAAAACATTC 1 22.97

Total number of rows: 15922

Table truncated, full table size 297 Kbytes.




Supplementary data files not provided

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