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Sample GSM384400

Query DataSets for GSM384400

Status

Public on Mar 20, 2009

Title

mSAGE_T_cell_tolerated_skin_graft_normal_CL

Sample type

SAGE

Anchor

NlaIII

Tag Count

22096

Tag Length

Source name

t-cell, normal, cell line, pcr directed strategy, cdna template, short sage

Organism

Mus musculus

Characteristics

organ/tissue: white blood cells
tissue preparation: cell line
sex: unknown
other information: A CD4+ Treg/Tr1-like H-Y + H2Ek specific line grown from the accepted male skin grafts of female A1(M) TCR transgenic mice. mRNA was derived from CD4+ T cells (>99%) 7 days from last stimulation, and contains <0.1% mast cells. cDNA was preamplified (SMART) before SAGE analysis.[SAGEMap name: Treg line SKA from skin graft]

Extracted molecule

total RNA

Extraction protocol

N/A

Description

Tagging_enzyme: BsmF I
CGAP_Numeric_library_ID: 1067
Laboratory: Diana Zelenika, Elizabeth Adams, Susan Humm, Luis Graca, Sara Thompson, Steve Cobbold, Herman Waldmann

Submission date

Mar 19, 2009

Last update date

Mar 20, 2009

Contact name

Cancer Genome Anatomy Project CGAP

E-mail(s)

cgapbs-r@mail.nih.gov

Phone

301-496-1550

URL

http://cgap.nci.nih.gov/

Organization name

National Cancer Institute

Department

Cancer Genome Anatomy Project

Street address

City

Bethesda

State/province

ZIP/Postal code

20852

Country

USA

Platform ID

GPL11

Series (2)

GSE14	Cancer Genome Anatomy Project (CGAP) SAGE Libraries
GSE15311	Cancer Genome Anatomy Project (CGAP) Mouse SAGE

Data table header descriptions
TAG
COUNT
TPM	tags per million

Data table
TAG	COUNT	TPM
AAAAAAACTA	2	90.51
AAAAAACAAA	2	90.51
AAAAACACAG	1	45.26
AAAAACAGTG	1	45.26
AAAAACATCG	1	45.26
AAAAACCAAA	2	90.51
AAAAACTCTA	1	45.26
AAAAACTTCG	1	45.26
AAAAACTTGG	1	45.26
AAAAACTTTT	1	45.26
AAAAAGTACC	2	90.51
AAAAATAAAA	1	45.26
AAAAATATCG	2	90.51
AAAAATCATC	8	362.06
AAAAATCCAT	1	45.26
AAAAATGTGG	1	45.26
AAAACAAGCC	1	45.26
AAAACACGTG	1	45.26
AAAACAGGGC	1	45.26
AAAACAGTGC	3	135.77

Total number of rows: 7959

Table truncated, full table size 149 Kbytes.

Supplementary data files not provided