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Sample GSM38496 Query DataSets for GSM38496
Status Public on Jan 05, 2005
Title Pituitary, Male (MoPi.Male)
Sample type MPSS
 
Source name Pituitary, Male total RNA
Organism Mus musculus
Extracted molecule total RNA
 
Description Strain: C57BL6
Pituitary, Male total RNA (MoPi.Male) received from National Human Genome Research Institute was analyzed through Bioanalyzer and passed our quality control test. The mRNA was processed according to the MPSS protocol as outlined in the previous publications (Brenner, S., et al. (2000) Proc Natl Acad Sci U S A 97(4): 1665-1670 and Brenner, S. et al., Nat. Biotechnol 18(6): 630-634). Briefly, the mRNA was reverse transcribed and the cDNA was digested with Dpn II. The 20 bases adjacent to the 3? most Dpn II site was cloned into a Megaclone vector. The resulting library was amplified and loaded onto microbeads. About 1.6 million microbeads were loaded into each flow cell and the signature sequences were determined by a series of enzymatic reactions as outlined in the above publications. The abundance for each signature was converted to transcripts per million (tpm) for the purpose of comparisons between samples.
Cells/tissue:
Library MoPi.Male.sig22
Cell type Pituitary, Male
Source NHGRI
RNA isolation LYNX mRNA QC passed
cDNA library:
Library DpnII restriction - (signature cloning using MmeI)
Sequence length 20 bp
MPSS:
runs
MoPi.Male_sig22.5253W.g-20 12/21/2004 458280 10125 QC Passed
MoPi.Male_sig22.5253W.e-20 12/5/2004 316417 6378 QC Passed
MoPi.Male_sig22.5253F.d-20 12/3/2004 396477 3789 QC Passed
MoPi.Male_sig22.5253F.c-20 11/30/2004 367465 3747 QC Passed
MoPi.Male_sig22.5253W.d-20 11/30/2004 251051 6468 QC Passed
MoPi.Male_sig22.5253F.b-20 11/15/2004 270215 2094 QC Passed
Run group:
Total Beads successfully sequenced - 2059905
Processed Signatures - 10905
 
Submission date Jan 05, 2005
Last update date Dec 14, 2005
Contact name Daixing Zhou
E-mail(s) dzhou@solexa.com
Phone 510-670-9441
Fax 510-670-9302
URL http://www.solexa.com
Organization name Solexa
Department Bioinformatics
Street address 25861 Industrial Blvd
City Hayward
State/province CA
ZIP/Postal code 94545
Country USA
 
Platform ID GPL1010
Series (1)
GSE1581 MPSS mouse transcriptome analysis project

Data table header descriptions
ID_REF Signature - 20 nt sequence including DpnII site (GATC)
VALUE Mean abundance for a signature derived from all MPSS runs for the sample, in transcripts per million (TPM)
ST_DEV Standard deviation of the mean abundance from multiple MPSS runs
REP Replication_global


Total number of rows: 6313

Table truncated, full table size 189 Kbytes.




Supplementary file Size Download File type/resource
GSM38496.raw.txt.gz 157.8 Kb (ftp)(http) TXT

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