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Sample GSM385540 Query DataSets for GSM385540
Status Public on May 01, 2009
Title CDR021
Sample type RNA
 
Source name cardiac left ventricle
Organism Mus musculus
Characteristics sex: male
strain: C57BL/6J (or B)
surgery: castration
Extracted molecule total RNA
Extraction protocol Total RNA was extracted, using the RNeasy minikit (Qiagen Canada, Mississauga, ON), from cardiac left ventricles obtained from 12 week-old male mice.
Label biotin
Label protocol Biotinylated probes were prepared from 50 ng of total RNA, using the Ambion Illumina TotalPrep RNA Amplification kit (Applied Biosystems, Streetsville, ON).
 
Hybridization protocol Standard Illumina hybridization protocol
Scan protocol Standard Illumina scanning protocol
Description replicate 1
Data processing Variance was stabilized by log2 transformation, and data were normalised using quantile normalisation with lumi in R
 
Submission date Mar 23, 2009
Last update date Mar 23, 2009
Contact name Christian F Deschepper
E-mail(s) deschec@ircm.qc.ca
Phone 514 987 5759
Fax 514 987 5585
Organization name IRCM
Lab Cardiovascular biology
Street address 110 ave des Pins Ouest
City Montreal
State/province QC
ZIP/Postal code H2W1R7
Country Canada
 
Platform ID GPL6885
Series (1)
GSE15354 Cardiac left ventricles of 12 week-old male C57BL/6J and C57BL/6J-chrY<A/J/NaJ> mice

Data table header descriptions
ID_REF
VALUE quantile normalized log2(average signal)

Data table
ID_REF VALUE
ILMN_1250052 6.349018
ILMN_3122480 7.593235
ILMN_2599935 6.878217
ILMN_2675543 8.751069
ILMN_2686883 7.064228
ILMN_2751818 5.950016
ILMN_2728634 13.04401
ILMN_3040515 6.594848
ILMN_2711608 6.113488
ILMN_1232875 6.247952
ILMN_1258507 6.414655
ILMN_2746142 8.679324
ILMN_1252690 6.108171
ILMN_2655499 13.07981
ILMN_1252870 8.549853
ILMN_1248179 6.519778
ILMN_2649955 6.177744
ILMN_2628708 10.98249
ILMN_3024781 5.980947
ILMN_2705628 8.227116

Total number of rows: 25697

Table truncated, full table size 549 Kbytes.




Supplementary data files not provided
Processed data included within Sample table
Raw data are available on Series record

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