Total RNA was extracted, using the RNeasy minikit (Qiagen Canada, Mississauga, ON), from cardiac left ventricles obtained from 12 week-old male mice.
Label
biotin
Label protocol
Biotinylated probes were prepared from 50 ng of total RNA, using the Ambion Illumina TotalPrep RNA Amplification kit (Applied Biosystems, Streetsville, ON).
Hybridization protocol
Standard Illumina hybridization protocol
Scan protocol
Standard Illumina scanning protocol
Description
replicate 4
Data processing
Variance was stabilized by log2 transformation, and data were normalised using quantile normalisation with lumi in R
