NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM385576 Query DataSets for GSM385576
Status Public on Dec 31, 2010
Title EpiSCf, rep1
Sample type RNA
 
Source name EpiSC OG2 female
Organism Mus musculus
Characteristics gender: female
Extracted molecule total RNA
Extraction protocol 300 ng of total DNA-free RNA was used as input for labelled cRNA synthesis (Illumina TotalPrep RNA Amplification Kit - Ambion) following the manufacturer's instructions (IVT: 10h).
Label biotin
Label protocol Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays.
 
Hybridization protocol Standard Illumina hybridization protocol.
Scan protocol Standard Illumina scanning protocol.
Description replicate 1 of 2.
Data processing The bead intensities were mapped to gene information using BeadStudio 3.2 (Illumina), background correction was perform using the Affymetrix Robust Multi-array Analysis (RMA) background correction model, variance stabilization was performed using the log2 scaling and gene expression normalization was calculated with the quantile method implemented in the lumi package of R-Bioconductor.
 
Submission date Mar 23, 2009
Last update date Dec 31, 2010
Contact name Marcos J Ara├║zo-Bravo
E-mail(s) mararabra@yahoo.co.uk
Organization name Max Planck Institute for Molecular Biomedicine
Department Cell and Developmental Biology
Lab Computational Biology and Bioinformatics
Street address Rontgenstr. 20
City Muenster
ZIP/Postal code 48149
Country Germany
 
Platform ID GPL6885
Series (1)
GSE15358 Sox2 Levels Define Pluripotent Ground States and Reprogramming Potential

Data table header descriptions
ID_REF
VALUE Quantile normalized

Data table
ID_REF VALUE
ILMN_1212607 3.939482
ILMN_1212612 2.351697
ILMN_1212619 4.632856
ILMN_1212628 2.241514
ILMN_1212632 4.526045
ILMN_1212636 11.616283
ILMN_1212637 9.832014
ILMN_1212645 5.412302
ILMN_1212648 7.353409
ILMN_1212653 2.662405
ILMN_1212672 8.671000
ILMN_1212682 3.902924
ILMN_1212683 2.035213
ILMN_1212685 2.026340
ILMN_1212692 5.621505
ILMN_1212693 3.940153
ILMN_1212695 2.315571
ILMN_1212698 2.009364
ILMN_1212702 3.673987
ILMN_1212703 5.004399

Total number of rows: 25697

Table truncated, full table size 554 Kbytes.




Supplementary data files not provided
Raw data provided as supplementary file
Processed data included within Sample table
Raw data are available on Series record

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap