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Sample GSM385583 Query DataSets for GSM385583
Status Public on Dec 31, 2010
Title P19-Sox2, rep2
Sample type RNA
 
Source name P19 embryonic carcinoma cell overexpressing Sox2
Organism Mus musculus
Characteristics gender: male
Extracted molecule total RNA
Extraction protocol 300 ng of total DNA-free RNA was used as input for labelled cRNA synthesis (Illumina TotalPrep RNA Amplification Kit - Ambion) following the manufacturer's instructions (IVT: 10h).
Label biotin
Label protocol Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays.
 
Hybridization protocol Standard Illumina hybridization protocol.
Scan protocol Standard Illumina scanning protocol.
Description replicate 2 of 2.
Data processing The bead intensities were mapped to gene information using BeadStudio 3.2 (Illumina), background correction was perform using the Affymetrix Robust Multi-array Analysis (RMA) background correction model, variance stabilization was performed using the log2 scaling and gene expression normalization was calculated with the quantile method implemented in the lumi package of R-Bioconductor.
 
Submission date Mar 23, 2009
Last update date Dec 31, 2010
Contact name Marcos J Ara├║zo-Bravo
E-mail(s) mararabra@yahoo.co.uk
Organization name Max Planck Institute for Molecular Biomedicine
Department Cell and Developmental Biology
Lab Computational Biology and Bioinformatics
Street address Rontgenstr. 20
City Muenster
ZIP/Postal code 48149
Country Germany
 
Platform ID GPL6885
Series (1)
GSE15358 Sox2 Levels Define Pluripotent Ground States and Reprogramming Potential

Data table header descriptions
ID_REF
VALUE Quantile normalized

Data table
ID_REF VALUE
ILMN_1212607 3.182721
ILMN_1212612 2.894611
ILMN_1212619 5.494789
ILMN_1212628 2.753021
ILMN_1212632 4.788798
ILMN_1212636 12.291191
ILMN_1212637 9.252594
ILMN_1212645 4.592901
ILMN_1212648 7.389955
ILMN_1212653 5.146846
ILMN_1212672 10.073797
ILMN_1212682 4.113572
ILMN_1212683 2.494934
ILMN_1212685 1.944163
ILMN_1212692 6.476891
ILMN_1212693 4.802719
ILMN_1212695 2.359208
ILMN_1212698 2.687985
ILMN_1212702 2.778086
ILMN_1212703 5.715673

Total number of rows: 25697

Table truncated, full table size 554 Kbytes.




Supplementary data files not provided
Raw data provided as supplementary file
Processed data included within Sample table
Raw data are available on Series record

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