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Sample GSM385587 Query DataSets for GSM385587
Status Public on Dec 31, 2010
Title EpiSox2mL2, rep2
Sample type RNA
Source name EpiSC GOF18 male overexpressing Sox2 cultured in condition EpiSC medium (CM)
Organism Mus musculus
Characteristics gender: male
Extracted molecule total RNA
Extraction protocol 300 ng of total DNA-free RNA was used as input for labelled cRNA synthesis (Illumina TotalPrep RNA Amplification Kit - Ambion) following the manufacturer's instructions (IVT: 10h).
Label biotin
Label protocol Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays.
Hybridization protocol Standard Illumina hybridization protocol.
Scan protocol Standard Illumina scanning protocol.
Description replicate 2 of 2.
Data processing The bead intensities were mapped to gene information using BeadStudio 3.2 (Illumina), background correction was perform using the Affymetrix Robust Multi-array Analysis (RMA) background correction model, variance stabilization was performed using the log2 scaling and gene expression normalization was calculated with the quantile method implemented in the lumi package of R-Bioconductor.
Submission date Mar 23, 2009
Last update date Dec 31, 2010
Contact name Marcos J Ara├║zo-Bravo
Organization name Max Planck Institute for Molecular Biomedicine
Department Cell and Developmental Biology
Lab Computational Biology and Bioinformatics
Street address Rontgenstr. 20
City Muenster
ZIP/Postal code 48149
Country Germany
Platform ID GPL6885
Series (1)
GSE15358 Sox2 Levels Define Pluripotent Ground States and Reprogramming Potential

Data table header descriptions
VALUE Quantile normalized

Data table
ILMN_1212607 2.376827
ILMN_1212612 2.900813
ILMN_1212619 5.582852
ILMN_1212628 2.360767
ILMN_1212632 4.686474
ILMN_1212636 11.811483
ILMN_1212637 7.913068
ILMN_1212645 6.039579
ILMN_1212648 7.741651
ILMN_1212653 3.316999
ILMN_1212672 7.863633
ILMN_1212682 2.213821
ILMN_1212683 1.622946
ILMN_1212685 2.696516
ILMN_1212692 8.027090
ILMN_1212693 5.288525
ILMN_1212695 2.634044
ILMN_1212698 2.979225
ILMN_1212702 6.698004
ILMN_1212703 4.366470

Total number of rows: 25697

Table truncated, full table size 554 Kbytes.

Supplementary data files not provided
Raw data provided as supplementary file
Processed data included within Sample table
Raw data are available on Series record

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