|
| Status |
Public on Mar 27, 2009 |
| Title |
E6.5 retina RCAS Wnt2b rep1 |
| Sample type |
RNA |
| |
|
| Source name |
E6.5 retina expressing Wnt2b (1)
|
| Organism |
Gallus gallus |
| Characteristics |
protocol: expression of Wnt2b
age: E6.5
tissue: retina
|
| Extracted molecule |
polyA RNA |
| Extraction protocol |
Tissues were homogenized with Trizol reagents.
Total RNA was purified following the manufacturer's instruction.
polyA RNA was purified on Oligo-dT column.
|
| Label |
3' amplification Reagents for IVT
|
| Label protocol |
Labeling reaction was carried out following the manufacturer's instruction.
|
| |
|
| Hybridization protocol |
Hybridization was carried out following the manufacturer's instruction.
|
| Scan protocol |
Scan was carried out following the manufacturer's instruction.
|
| Description |
E1.5 retinas were electroporated with RCAS Wnt2b. Retinas were recovered at E6.5.
Wnt2b inhibits neuronal differentiation
|
| Data processing |
Affymetrix GCOS 1.4
|
| |
|
| Submission date |
Mar 25, 2009 |
| Last update date |
Sep 23, 2010 |
| Contact name |
Shinichi Nakagawa |
| E-mail(s) |
nakagawas@riken.jp
|
| Phone |
+81-48-467-4227
|
| Fax |
+81-48-467-4517
|
| Organization name |
RIKEN ASI
|
| Lab |
Nakagawa Initiative Research Unit
|
| Street address |
Hirosawa 2-1
|
| City |
Wako |
| State/province |
Saitama |
| ZIP/Postal code |
351-0198 |
| Country |
Japan |
| |
|
| Platform ID |
GPL3213 |
| Series (1) |
| GSE15382 |
Gene expression profile of embryonic retinas expressing c-hairy1, Delta-1, or Wnt2b |
|
