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Sample GSM386252 Query DataSets for GSM386252
Status Public on Mar 26, 2009
Title PBMCs vehicle treated for 24 hours,replicate 3
Sample type RNA
 
Source name PBMCs vehicle treated for 24 hours,replicate 3
Organism Homo sapiens
Characteristics cell type: peripheral blood mononuclear cells
Treatment protocol Cells from donors 1-6 were grown in vehicle, the IC90 (0.2 uM R547) and 3xIC90 (0.6 uM R547) dose of R547 for 2 or 24 hours. Donor 7 was used as the untreated zero hour time-point
Growth protocol Human peripheral blood mononuclear cells (PBMCs) were separated from whole blood of seven healthy donors and cultured in six T75 flasks per donor at 10^7 cells per flask in 10% heat-inactivated FBS in RPMI medium
Extracted molecule total RNA
Extraction protocol Total RNA was extracted from cells using the Qiagen RNeasy Mini Kit
Label biotin
Label protocol RNA was converted into cDNA and cRNA according to the manufacturer’s recommendation and using manufacturer’s kits (Affymetrix, Santa Clara, CA). 15 ug of total RNA was converted to cDNA with the One-cycle cDNA Synthesis Kit with the addition of reagents from the Poly-A RNA Control Kit. cRNA was produced with the IVT Labeling Kit
 
Hybridization protocol Hybridization Mix contained, in addition to cRNA, reagents from the Hybridization Control Kit and Control Oligo B2. Staining and washing steps were performed as suggested by the manufacturer (Affymetrix, Santa Clara, CA).
Scan protocol Each hybridized Affymetrix GeneChip array was scanned with a GeneChip Scanner 3000 7G (Agilent/Affymetrix).
Description n/a
Data processing Affymetrix gene expression microarray data was first background corrected and normalized via the RMA procedure followed by QQ normalization (R/Bioconductor)
 
Submission date Mar 25, 2009
Last update date Aug 28, 2018
Contact name James Rosinski
E-mail(s) jim.rosinski@gmail.com
Organization name Roche
Department In Silico Sciences
Street address 340 Kingsland St
City Nutley
State/province NJ
ZIP/Postal code 07110
Country USA
 
Platform ID GPL570
Series (2)
GSE15389 Peripheral blood mononuclear cells treated with a CDK inhibitor
GSE15396 Peripheral blood mononuclear, DU145, and HCT116 cells treated with a CDK inhibitor
Relations
Reanalyzed by GSE119087

Data table header descriptions
ID_REF
VALUE QQ normalized RMA signal

Data table
ID_REF VALUE
1007_s_at 83.455406
1053_at 84.57296
117_at 142.513565
121_at 202.731171
1255_g_at 6.854066
1294_at 368.204407
1316_at 46.74992
1320_at 16.097641
1405_i_at 5306.61377
1431_at 9.302914
1438_at 22.574547
1487_at 140.187637
1494_f_at 29.071753
1552256_a_at 122.33461
1552257_a_at 191.673004
1552258_at 15.822377
1552261_at 20.99497
1552263_at 204.163544
1552264_a_at 367.727173
1552266_at 9.782119

Total number of rows: 54675

Table truncated, full table size 1108 Kbytes.




Supplementary file Size Download File type/resource
GSM386252.CEL.gz 4.6 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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