|
Status |
Public on Jun 11, 2019 |
Title |
WtPH: PGC-1a4 1 |
Sample type |
RNA |
|
|
Source name |
primary hepatocytes
|
Organism |
Mus musculus |
Characteristics |
strain: C57BL/6J tissue: liver cell line: WtPH genotype/variation: protein variant cell type: primary mouse hepatocytes isoform: PGC-1a4 treatment: vehicle (PBS)
|
Treatment protocol |
Adenoviral infection (48 hours) followed by treatment for two hours with 2 ng/mL TNFalpha
|
Growth protocol |
25 mM glucose DMEM supplemented with 0,2% BSA, 2 mM sodium pyruvate, and pen/strep.
|
Extracted molecule |
total RNA |
Extraction protocol |
RNeasy Kit column extraction
|
Label |
biotin
|
Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
|
|
Hybridization protocol |
standard Affymetrix procedure.
|
Scan protocol |
standard Affymetrix procedure.
|
Description |
microarray expression data from primary hepatocytes
|
Data processing |
The data were analyzed with RMA (log2) normalized data using the just.rma function in the affy package in Biocinductor
|
|
|
Submission date |
Jun 10, 2019 |
Last update date |
Jun 11, 2019 |
Contact name |
Jennifer Lynn Estall |
E-mail(s) |
jennifer.estall@ircm.qc.ca
|
Phone |
514-987-5688
|
Organization name |
IRCM
|
Street address |
110 avenue des Pins ouest
|
City |
Montreal |
State/province |
Quebec |
ZIP/Postal code |
H2W1R7 |
Country |
Canada |
|
|
Platform ID |
GPL1261 |
Series (1) |
GSE132458 |
Effects of Peroxisome proliferator-γ coactivator-1α (PGC-1a) isoform over-expression +/- TNFalpha on hepatocyte gene expression |
|